We narrowed to 996 results for: ran
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TypeBlog Post...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS...
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Addgene Depositors Get More Citations
TypeBlog Post...on a range of topics including biotech and research tools, computing performance, and randomized control... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, ... -
Plasmids 101: Screens vs. Selections
TypeBlog Post... a function in a given process. The process can range from a defined pathway or reaction to a complex ...phenotypical outcome. In forward screens, there is random or unknown manipulation of the population. This... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...discussed and Professor Caetano Reis e Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy... Kettering Cancer Center. In contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome ... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...scientists and the support of their technology transfer offices, Addgene would not be able to have such... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...induces the DNA base change without a DNA double-strand break. But base editors aren’t perfect. They may...References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base ... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...example, matches of a name in the depositor field will rank higher than matches of the same name in the publication... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...native endonuclease activity degrades the displaced strand. Figure 1: EvolvR consists of an error-prone...lineage tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...at both MetLife & Ernst & Young, LLP, auditing insurance, finance and high-tech industries. He has earned... systems auditor (CISA), and certified health insurance executive (CHIE). He is a member of both the Internal... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...work for almost 20 years. It is good to be a bit random because you never know which relationships will...These are all normal outcomes. If you have a broad range of mentors you will always have people you can turn... -
Antibodies 101: Affinity Reagents
TypeBlog Post...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...an excellent option for many applications, and frankly, if ain't broke, don't fix it! That being said... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...-independent assays such as mRNA data from transcriptomics; independent antibody strategies that compare...validation does not mean that the antibody is guaranteed to work. Antibody-based assays are complex and... -
Developing Lab Management Software for Biology
TypeBlog Post... lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly left...t have a system through which she could easily transfer her instructions for dealing with the samples ... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...expression of other genetic elements including transcription factors (Cheng et al., 2014) and microRNAs (.... Konermann, Silvana, et al. "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-linear dependence on excitation...the conclusions reached at high power may not translate to real applications in which far less power is... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...was also recently awarded a Mozilla Science mini-grant, which will fund a project to support a dedicated...on the Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about ...