We narrowed to 838 results for: tat

1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...

2. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   ...protein in low abundance or whose conformational state may change. If your protein might become slightly...

3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...

4. Developing Lab Management Software for Biology

   Type

   Blog Post

   ... track the location, quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   ...Gendered Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and...

6. Plasmids 101: Modular Cloning

   Type

   Blog Post

   ... allows users to test a variety of different orientations and components within the system to maximize...

7. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   ...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...

8. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   ...that require high affinity binding, such as immunoprecipitations. Your sample species should also be considered...

9. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

10. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    ...without clearly testing whether a certain methylation state causes a condition. To understand mechanisms and...

11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

12. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...ratio for the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions...

14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...

15. CRISPR in the Clinic

    Type

    Blog Post

    ...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

16. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    ... to a target cell by reducing the repulsive electrostatic forces between these two negatively-charged ...

17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...

18. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    ...sequence conserved Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English...

19. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    ...use any slides for the event. Slides are for presentations, not discussions. I will rarely work with a ...

20. Hot Plasmids: Winter 2025

    Type

    Blog Post

    ... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...