We narrowed to 847 results for: tat

1. Plasmids 101: The protein expression toolbox

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   Blog Post

   ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

2. All in a Twist: dsRNA

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   Blog Post

   ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...

4. Antibodies 101: Polyclonal Antibodies

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   Blog Post

   ...protein in low abundance or whose conformational state may change. If your protein might become slightly...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   ...modeling. Its reversibility allows precise and quantitative analysis of epigenetic memory at nearly any ...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...

7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

   ...let’s take a look at exosomes. Proficient in transportation, exosomes are tools of communication and provide...

8. CRISPRainbow and Genome Visualization

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   Blog Post

   ...fluorescently labeled Cas9’s come with a number of limitations. Since color is specified by PAM sequence, each...

9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   Blog Post

   ...actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against...

10. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are even going...

11. Developing Lab Management Software for Biology

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    Blog Post

    ... track the location, quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene...

12. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

    ...Gendered Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and...

13. Plasmids 101: Modular Cloning

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    Blog Post

    ... allows users to test a variety of different orientations and components within the system to maximize...

14. CRISPR in the Clinic

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    Blog Post

    ...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

15. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...

16. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...that require high affinity binding, such as immunoprecipitations. Your sample species should also be considered...

17. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...without clearly testing whether a certain methylation state causes a condition. To understand mechanisms and...

19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

20. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...