We narrowed to 916 results for: tro

1. Management for Scientists: Delegating is Key

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   Blog Post

   ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ...

2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...background on fluorescence and filter sets, see the Introduction to Fluorescence Microscopy lecture at iBiology...

3. Fluorescent Proteins 101: Luciferase

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   Blog Post

   ...luciferase gene in the plasmid, which is then introduced into the desired mammalian, plant or bacterial...

4. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...They might be researching enhancer elements that control the transcription of a gene a megabase away, or...

5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...genome, and showed success in models of muscular dystrophy. In this case, Konermann et al. targeted MAPT,...

6. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ...more open way of communicating in science brings stronger networking across laboratory and disciplinary ...

7. How Addgene Creates Content

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   Blog Post

   ...  Susanna Stroik...

8. Plasmids 101: Screens vs. Selections

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   Blog Post

   ... Susanna Stroik...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...Because the performance of a fluorescent protein strongly depends on both the biological system as well ...

10. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...

11. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...-Cas9 tools have been used in immunology for in vitro screens of immune cell activity. However, a CRISPR-based...

12. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ...more about research applications for antibodies in Intro to Antibodies Read about the Developmental Studies...

13. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation...

14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...both small and potentially less likely to elicit a strong immune response, factors that may make it especially...

15. Hot Plasmids - May 2022

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    Blog Post

    ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

16. Hot Plasmids - February 2022

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    Blog Post

    ...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

17. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    ...  Susanna Stroik...

18. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...

19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...equipment that still works. “We use functional spectrometers from the early 1990s that were free and free...

20. Visualizing Protein Turnover In Situ

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    Blog Post

    ...protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...