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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: ins;

Showing: 401 - 410 of 1045 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... things like genomic DNA, RNA, proteins, salts, ethanol, or endotoxins. The most common method for checking...to be free of contaminants, such as bacterial endotoxins, that could affect your culture’s viability. ...plasmids – If you’re extracting plasmids from yeast, instead of bacteria, a few manufacturers (such as Zymo ...struggling with a purification, reading the full instruction manual will help explain the rationale behind... helpful to select a kit with easy-to-follow instructions and extensive references for troubleshooting... compare kits at a quick glance, Table 3 below contains details regarding kits from a variety of manufacturers...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

  2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...NanoDrop. The principles will apply to similar instruments from other companies, like the Implen NP80 or...Figure 1A). Thanks to the Beer–Lambert law, the instrument can calculate a sample’s nucleic acid concentration...downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own... trying to remove from your sample is protein. Proteins absorb light to varying degrees across the UV ...Cantor, 2019). The 280 nm peak is more specific to proteins and why researchers traditionally use the A260...will yield “good” A260/280 and A260/230 ratios. Instead, phenol is more likely to shift the peak of your...! Be sure to review resources on the specific instrument you use and check out some of the references ...

  3. Deck the Lab 2022!

    Type
    Blog Post
    ... contestants!           View this post on Instagram                     A post shared by Xi Chen...December 12, 2022          View this post on Instagram                     A post shared by 草方 (@gracie_fang...take photos, and then share them on Twitter or Instagram with #DeckTheLab and tagging us, @addgene to be...get you out of.           View this post on Instagram                     A post shared by Xi Chen...picture or video for either contest on Twitter or Instagram by December 31, 2022.  Follow @Addgene, use the...and reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light...

  4. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...diagnosed this year). Research efforts in mesothelioma instead rely upon private donations. Many people think ...However, there is no question that asbestos exposure remains the single-most important factor in developing ...do not work very well. The best option in such instances is palliative care, which helps patients manage...detection techniques and therapies emerge into mainstream use, they will have a much greater impact on ... good detection and treatment get, there still remains the problem of prevention. Asbestos exists pretty... and the president of the organization, Linda Reinstein – whose husband passed away from mesothelioma ...with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016 in the U.S. Senate...

  5. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...frequency of events, instead of relying on clustered dots. In a contour plot, each line contains the same number...machine reads/interprets the signals, the axis is instead divided into positive and negative signal at a ... a ‘gate’; it tells the machine that everything inside the gate is one population and everything outside...which measure light that “scatters” past the cell, instead of fluorescent markers. There are various types...to fluorescent markers and/or cellular dyes and stains. These will be used in a series of one- or two-...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels (which had therefore been ... light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential labeling by TurboID...2023 bioRxiv (B-C). They were able to label proteins moving to and from the nucleus, ER, mitochondria...

  7. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...reprogramming efficiency through the expression of a shRNA against p53, a modified reprogramming factor cocktail, ...are preferred. This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes...disruption of functional genes at the site of insertion and which can potentially promote tumorigenesis... been recorded in past gene therapy trials and remains a safety concern in the clinical field [4]. Three...Antigen-1 (oriP-EBNA-1) system where each vector contains a viral origin of DNA replication, OriP and the... pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1 37624 transient EBNA-1   ...Road Ahead." Journal of Alzheimer's disease & Parkinsonism 6.5 (2016). PubMed PMID: 27882265. PubMed Central...

  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... apply for the position. This post on Business Insider nicely summarizes good job fair practice. Career...get online and take a look at the company or institution’s career page, where you’re likely to find current...conference or job fair, or it may be someone at your institution’s career center. Alternatively, it might even ...other graduate students and post-docs from your institution have gone, and try sending them a professional... professional development, not count on your institution, program, or adviser to do it for you. Expose...if one exists. Get online! Review company and institution career pages. Make yourself visible on LinkedIn... Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014...

  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Singletons in their bins.  The next component of Binning Singletons is when we bring all the bins together at ...invaluable to me as a bench scientist working at an institution where training opportunities weren’t always available...their own labs.  Fried chicken with a side of inspiration  I look back at the meeting with such fondness...for DNA that group similar sequences together in bins, we thought that binning Singletons together would... and time mentors spend with Singletons in their bins. We also sought expertise from many with experience...media. Joe is a lapsed marathon runner, father of twins (AND a singleton), and is proudly "too weird to ...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...DNA left behind after another bacterium died for instance). These three mechanisms of acquired drug resistance...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be... time is needed for the drug to work… if the instructions for treatment aren’t followed closely, you could...in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our Microbiology Pages Additional...
Showing: 401 - 410 of 1045 results