Skip to main content
Addgene
Showing: 801 - 820 of 1008 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG...

  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... a downregulation of signal, though it’s hard to call from this plot. The red (bottom) peak overlaps with...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

  5. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... the following, which are later printed for my physical lab notebook, along with any figures generated...between each word can make it difficult to accurately call a file. Version control your commonly used scripts...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...once it is engaged by its target, it will non-specifically cut ssDNA. This feature is shared by other Cas... potential as a viral therapeutic that could specifically degrade target viral RNA. For more information...

  8. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...out the sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably well...answer to these questions--which were real, not hypothetical--was clearly “no.” When there was room for misinterpretation...

  9. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...library, propagated it, separated it into roughly identical replicate groups, and treated these groups with...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

  10. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...Another strength of Golden Gate cloning is its scalability. Unique 4 base overhangs can be used to assemble...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...

  11. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ... will provide an initial consultation meeting or call for free.  Take advantage of this to get the information...blogged for us) offers a free report on her website called “Secrets to Success in Graduate School”. She has...

  12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...characters. A descriptive purpose focuses on the practical application of the plasmid and will help a scientist... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... for PAM-less editing   Guides for CRISPR are typically selected by the ‘quality’ of the gRNA sequence...the Liu lab through phage-assisted evolution, specifically with the goal of generating Cas enzymes with...

  15. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...search algorithm rather than pre-designed sets, so identical searches can return multiple answers. You might...might want to take advantage of their save and recall functions when you get results you like. NEBridge ...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    ... gRNA (Anzalone et al., 2019), disrupting its canonical structure and Cas9 binding (Nishimasu et al., ...other bases in this location bind to Cas9 with canonical sgRNA structure (right). Created with BioRender.com...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...to visualize DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing...

  20. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...sequence (gene). In a clean sample, there are typically fewer than 10 hits to a given sequence, and we...control is recommended but not yet required for clinical-grade materials, and is not commonly performed...
Showing: 801 - 820 of 1008 results