We narrowed to 994 results for: Des

1. 2023 and 2024: Reflections and Looking Ahead

   Type

   Blog Post

   ...accessibility updates include a printer-friendly design and alt text on all images. We have also made accessibility...

2. New Norepinephrine Indicators: nLightG and nLightR

   Type

   Blog Post

   ...will need to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG...

3. Supporting Rare Disease Research with BeHeard 2017

   Type

   Blog Post

   ...rare diseases. These underrepresented individuals deserve a voice. Check back with Addgene often to learn...

4. Mapping the 4D nucleome with CRISPR/Cas9

   Type

   Blog Post

   ...PAM sequences required by each ortholog, a gRNA designed for one dCas9 should be specific to that ortholog...

5. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   ...multiple recombinases can be used together to designate specific patterns of recombination. Finally, Rubin...

6. Future of Research Conference - Remarkable Opening Session

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   Blog Post

   ...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...

7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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   Blog Post

   ...with genes knocked out in the insulin signaling cascades in only 4 months compared to a year or greater...

8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   Blog Post

   ...already made for them,” Baird said. “They are all designed in the same vector backbone with very similar ...

9. London Calling - Addgene’s New Europe Office in London

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   Blog Post

   ...deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists...

10. 9 Tips to Achieve Success in Academia

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    Blog Post

    ...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...

11. What is Polymerase Chain Reaction (PCR)

    Type

    Protocol

    ...Gel Procedure Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...the denaturing. Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set...protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do if my... to work efficiently. Taq Buffer with MgCl 2 : Provides an optimal and stable chemical environment for...

12. Viral Production

    Type

    Collection

    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...

13. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    Blog Post

    ...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

15. Fostering communication in remote collaborations

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    Blog Post

    ...and morale. Especially when your collaboration includes scientists working in different time zones, it...

16. We're Updating Our Hold for Publication Policy

    Type

    Blog Post

    ... release plasmids on hold if they are clearly described in a journal publication. Plasmids will be released...

17. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type

    Blog Post

    ... to succeed. As a team, we talked through slide design, discussion items, big takeaways, and timing. The...

18. CRISPR 101: Drug discovery and CRISPR

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    Blog Post

    ...onset) may be best served by this type of targeted design. Alternatively, a disease model can be generated...

19. Plasmids 101: Gene, enhancer, and promoter trapping

    Type

    Blog Post

    ...Each trap uses a slightly different approach (described in detail below), but fundamentally the reporter...

20. Bringing Sustainable Practices to the Lab: Recycling

    Type

    Blog Post

    ...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ...