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...oligo overlap cloning, you can design a set of oligos containing your desired restriction sites and add them...that will pay off for years to come. Design Briefly, we will design overlapping oligos that once annealed...procedure will simply differ in terms of primer design). Let's assume that your favorite vector has a ...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...between the EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in...complementary C of the other oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

... brand friendly. I introduced the team to a new design program, Canva, and I was able to create cute and...

...only be expressed in neurons. Indeed, this AAV provides you with a means to specifically activate expression...

... blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog series will help...

...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...

...     Science’s John Bohannon published an article describing a sting operation in which he submitted a phony...

...Introductory techniques designed to help you get started in the lab. Name Description (Link opens in a new...gel Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...blocks for many more complicated procedures. Name Description (Link opens in a new window) Link to Video Making...constructing and analyzing your plasmids. Name Description (Link opens in a new window) Link to Video Restriction...titering and testing your virus preparations. Name Description Link to Video General Transfection Introduce ...Protocols for common antibody applications. Name Description Link to Video Transfection Introduce plasmid ...

... that 2014 will be a year of lab BBQ parties - despite BBQs being wishful thinking in England. The Cepko...

...rare diseases. These underrepresented individuals deserve a voice. Check back with Addgene often to learn...

...internationally COVID-19 Resources Canada is a database that provides information about reagents, funding and volunteer...

... whose expression varies depending on transgene design and delivery. They generated hPSC lines with AAVS1...

...accessibility updates include a printer-friendly design and alt text on all images. We have also made accessibility...

...will need to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG...

...PAM sequences required by each ortholog, a gRNA designed for one dCas9 should be specific to that ortholog...

...multiple recombinases can be used together to designate specific patterns of recombination. Finally, Rubin...

...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...

...with genes knocked out in the insulin signaling cascades in only 4 months compared to a year or greater...

...already made for them,” Baird said. “They are all designed in the same vector backbone with very similar ...

...deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists...