We narrowed to 856 results for: HA

1. Hot Plasmids - August 2020

   Type

   Blog Post

   ...with low affinity variants of TEV. The new system has been demonstrated to work for evolution of other ...

2. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ... original CAPTURE method is that a new cell line has to be created for each genomic loci of interest. ...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...how to create the iodixanol gradient layers, and has some great pointers that while help you master iodixanol...

4. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ..., TOP10, and Mach1. No common lab cloning strain has failed so far. What is most important is the transformation...

5. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...

6. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   ...what to delegate and to whom (make sure that person has time for it!). Then schedule a meeting to discuss...

7. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   ...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...

8. Bioinformatics at Addgene

   Type

   Blog Post

   ... and continuous development of in-house software has been crucial for us to keep pace with our current...

9. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   ...be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool...

10. Design Tips for Prime Editing

    Type

    Blog Post

    ...prevents the prime editor from re-engaging with DNA it has already edited. Created with BioRender.com. ...

11. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ...sample, called a standard. The ideal standard curve has at least five dilutions, with each step of the series...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result...

13. How to Be an Excellent Trainee

    Type

    Blog Post

    ...the bench waiting for you, prep for research work has to be done by the researchers themselves.  Be ...

14. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious...

15. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    ...While the method is simple and saves time, it also has several drawbacks. Direct ELISAs generally have higher...

16. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing titering...

17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ...transcription template (RTT). The design of the pegRNA has been shown to play a critical role in the editing...

18. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ... from R1 institutions to community colleges, and has helped provide original research experiences for ...

19. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    ...There are many moving parts to our software, which has grown with the company. It would be easy to assign...

20. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ...two flanking repeats. In cells where recombination has not occured, methanol induces the production of the...