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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,082 results for: ica;

Showing: 801 - 820 of 1082 results

  1. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...Roth’s lab are already available allowing you to chemically control neuronal activity while studying your...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ... service as we continue to develop it. More communication and sharing will only help research advance....

  2. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...Chavez, researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene...achieving the gRNA representation recommended in the publications associated with these libraries. Please note...particular, the Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...physiology. Ribosome profiling Ribosome profiling, a biochemical method developed in 2009, takes a “snapshot” ...translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating RNA...development, the osk-TRICK reporter was double labeled, indicating translational repression. During later stages...studying translation in various cell types and physiological states. References 1. Ingolia, Nicholas T.,...

  4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post
    ...microorganisms based on metagenomic data. MerMAIDs (Metagenomically discovered Marine, Anion-conducting and Intensely...publicly available metagenomic datasets with biophysical studies and the potential of mining datasets ...plasmids at Addgene Opperman J et al., Nature Communications, 2019. PubMed PMID: 31346176. Using LADL to...CIBN), which they call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these ...

  5. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

  6. Hot Plasmids - May 2022

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    Blog Post
    ...of interest and, for in vivo work, generate a dedicated transgenic line. Using zebrafish as a model organism... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP...clonal multiplexing of cells in live imaging application. Each visual barcode consists of a fluorescent...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...neurons and specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules... to optogenetics but instead of light, these genetically modified receptors are activated by small molecules...some GPCRs further to respond to specific, pharmacologically-inert, small molecules. These new receptors...Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...

  8. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ... light-mediated? The researchers split the proteolytically active light chain of BoNT/B into two fragments...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...rely heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance,...the field. Arlene Sharpe’s group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9...

  9. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...notice the “V2.0” at the end of this plasmid which indicates that there was a prior version of this plasmid...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...generation lentiviral backbone that must be used with lentiCas9-Blast or with cell lines already expressing Cas9...

  10. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...study it. The process is fairly straightforward. Basically, you place your gene of interest in a plasmid ...genetic crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... aware of at least some of the possibilities. Basically, if you can devise an experimental assay that ...

  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow... and correlating them with tumor mutations and clinical responses. This platform has the potential to ...the use of optogenetic tools is expanding to immunological research. Here the Zhou group presented Opto-CRAC... et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer cell 30.1 (2016):...

  12. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...α-complementation. The system described above was put to practical use in the following way. Scientists engineered...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR... Jessica Welch...

  13. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...missing or damaged nucleotides that are common to biological sources of DSBs, and which need to be processed...generated in the course of repair by NHEJ are typically small (1-10 bp) but extremely heterogeneous. There.... NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank...Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829...

  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post
    .... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet...This is a chance to practice leadership and communication skills. The leader also provides materials such...present formally often enough) and give honest, critical feedback for improvement. Go to a science or career...

  15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...produce genetically modified cells in less than a month's time. Addgene: What are the implications of this...Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you to build a DNA-binding...diseases. We are knocking out genes that have been implicated in disease or, in cases where particular variants...

  16. Addgene: The First Twenty Years

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    Blog Post
    ...donating and receiving labs)” — Jim Woodgett, biomedical researcher at Lunenfeld-Tanenbaum Research Institute...reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a special thanks to the...decade. We did this the old-fashioned way: through physical outreach, visiting organizations, knocking on ...standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled...

  17. The time and cost required to make a plasmid

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    Blog Post
    ...time it takes to make a plasmid could increase dramatically if you are piecing together several PCR products...to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...The faster methods, like Gibson assembly, are typically more expensive, so you’ll have to find a balance...calculations for any of the numbers in this post is a mathematical story well beyond my ability to tell. But even...

  18. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor... protein, then these systems need to be site-specifically knocked in. This can be labor intensive and ...and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID...

  19. Developing Lab Management Software for Biology

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    Blog Post
    ...our second example we’ve added some simple but critical logic. Some steps fail “hard”, they reset the ...interchange them easily as needed. In this case, the physical considerations of the laboratory make object composition...science_name`. You'll need to decide given the technical details of the problem you're solving, but consistency...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're...

  20. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post
    ...choose well. What are their qualifications? Do they have certifications? How can they demonstrate their...help you. A good coach usually will be a clear communicator and make direct suggestions for improvement ...she is especially excellent in coaching for communication skills. Industry transition is also an area ...
Showing: 801 - 820 of 1082 results