We narrowed to 918 results for: plasmid

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose response.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and more.

Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.

Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses.

Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses.

Learn about different molecular cloning techniques, focusing on seven common cloning methods.

Learn all about plasmid pooled libraries, how to amplify and use them, and what types of experiments can be done with a pooled library.

Read our adeno-associated viral vector guide to learn about AAV vector components, production and common uses.

Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and the MoClo Plant Parts Kit.

Overview of Addgene's educational resources, including eBooks, science guides, videos, and protocols

Learn about bacterial and eukaryotic promoters, including transcription and the transcription complex.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Not sure where to start? Need a quick reference? Addgene's eBooks are here to help!

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Collection of guides that describe how popular scientific techniques work

Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems.