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We narrowed to 971 results for: lars;

Showing: 411 - 420 of 971 results

  1. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...incomplete or nonexistent. In the 21st century, many molecular biologists didn’t know the complete sequences ... This algorithm enables one of SnapGene’s most popular aspects - its ability to annotate a raw plasmid...source of common features was our collection of popular plasmid sequences. These plasmids contain features...beginning or end of the feature. With the increasing popularity of gene synthesis, many researchers now use codon-optimized...Scientist at SnapGene, and also a Professor of Molecular Genetics and Cell Biology at the University of...

  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...production can be a frustrating process that requires a large investment of time and resources for labs that don...Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository are used to... phenotype of interest. Optogenetic tools for similarly controlling neuronal activity with light will ... soon. We’re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent...AAVs are getting to the right cells using your particular delivery technique and conditions. Finally, we...

  3. Plasmid Cloning by PCR

    Type
    Blog Post
    ...cloned into a plasmid of interest. You can use similar processes to add overhangs to your insert of interest...primer design and the PCR process itself are very similar to those outlined in our restriction cloning post...based cloning     The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18 bases of the ORF, including the stop...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... immune cells. Dendritic cells (DCs) are particularly popular whole cell therapeutics because they can...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...through these various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is...production, and CTLA4 and CD protein blocking. Other molecular targets under research include TIM-3, LAG-3, STAT...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

  5. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...screening experiments, so the scale of the DNA prep is larger. And since the library contains many plasmids, ...amplification protocol, but the overall workflow is similar for most libraries. Here are the key points you... For libraries where the plasmids are all very similar sizes, like a gRNA library, this is less of a concern... hope you’ll agree with me that they look very similar to each other - that’s exactly what we want! At...your amplification. I hope this post has helped clarify some of the important considerations when using...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... You can also find a list of popular sequencing primers in our Molecular Biology Reference. Sometimes ...sequencing or the other techniques mentioned above. In molecular biology, cloning a gene into your plasmid is often...plasmid basics in our Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org... Learn more about Sanger sequencing Read the Molecular Biology Reference ...

  7. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ... norovirus. While this assay is specific to a particular strain of norovirus, it can be tailored for the...segment The human proteome contains a breadth of modular domains that facilitate protein-protein interactions... and mediate biochemical processes. One of the largest and best characterized of these is the Src homology... sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought...

  8. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...colleague who preferred detailed instructions and regular check-ins, which helped them stay on track and ...resource: connecting with mentors who have made similar transitions can provide you with insights, lessons...mentoring a junior colleague. Guiding an intern, in particular, served as a great "baby project," offering a...strengths. By encouraging alignment on goals and clarifying roles, I eased the tension and improved collaboration... his PhD and a couple of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs...

  9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...Type Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism...Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research More...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari...for your experiment Monoclonal antibodies are particularly useful when you want to ensure that the antibody... the antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...
Showing: 411 - 420 of 971 results