We narrowed to 991 results for: Des

... release plasmids on hold if they are clearly described in a journal publication. Plasmids will be released...

...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

...and morale. Especially when your collaboration includes scientists working in different time zones, it...

...should also be easily understood and consistent in design and layout. 2. Be concise in your writing  When...

...distributed more than 100 times. We hope these desktop trophies will serve as badges of honor for our ...

.... Traditional restriction cloning is one of the oldest of these techniques and is a multi-step process...

... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...Career: Doer, Manager, Leader” by Charlotte Beers, divides workers into three cycles: Doers, Managers and ...

...best? What 3 things worked least well? Manager provides feedback (may be the same as self-assessment or...

...research, SciFund is really motivated more by the desire to help close the widening gap between science ...

...oligo overlap cloning, you can design a set of oligos containing your desired restriction sites and add them...that will pay off for years to come. Design Briefly, we will design overlapping oligos that once annealed...procedure will simply differ in terms of primer design). Let's assume that your favorite vector has a ...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...between the EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in...complementary C of the other oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...

...activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to...

... A -C compatibility grouping is an arbitrary designation, and plasmids from the same incompatibility group...

...harder to build relationships when you are more desperate or needy so you can’t wait until you imminently...