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  1. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...reunions in various wildernesses and enjoys “three generations walk[ing] together in the Arboretum and other... all work and no play Although hard work and perseverance are required to achieve success in the lab, ...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining fluorescent...proteins, but also investigate protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn...

  3. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...Twitter lists were searched for scientific titles generated from the United States Standard Occupational Classification...tweeted science domain by scientists was Nature. Overall scientists tweet URLs with scientific domains <...

  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... E. coli MG1655 genome with an NGG PAM motif, averaging 19 targets per gene. The lab has previously used...

  5. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...this is vital to ensuring that our services help accelerate your research. We know you don’t have time to...answer that you’re looking for, or you just have a general question or concern about the answers we provide...

  8. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins...order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion Ready for some...

  10. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at very low levels due...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  16. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ... a learn a lot from these ASM pros and hope to leverage their expertise as we advance our mission of sharing...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries...

  17. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...aliquots could be stored indefinitely at room temperature (thus also saving freezer space).  The procedure...BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast... Taylor (Ed.), Viruses and Man: A History of Interactions (pp. 23–40). Springer International Publishing...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...
Showing: 821 - 840 of 998 results