We narrowed to 856 results for: HA

1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...something called “the photon budget”: each sample has a limited number of fluorophores and each fluorophore...

2. Lentiviral Vector Uses and Overview

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   ...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of...

3. CRISPR Antimicrobials

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   ...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...

4. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...measles, mumps, and rabies. Research on Ebola virus has been limited, in part, due to the necessity for working...

5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ...enabled numerous cell biology assays. However, there has been a pervasive view that most FPs in a cell will...

6. Plasmids 101: Control Plasmids

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   ...transfection reagents or the transfection process itself has any cytotoxic effects on the target cells. Another...

7. Negotiating Work and Life: How to Find the Joy

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   ...sometimes put work ahead of life Sometimes, work has to come first. That means you and/or your spouse ...

8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...

9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   ...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...

10. Transferable Skills Guide: External Collaborations

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    ...problem or a need for something, usually someone else has realized this too. Begin with informal conversations...

11. CRISPR 101: Validating Your Genome Edit

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    ...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...

12. Filming Science Videos in the Age of Social Distancing

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    ...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...

14. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...reports which POI band is stronger. The right gel has exactly the same POI bands as the left gel, but because...

15. Twenty Years of Sharing: Expanding Accessibility

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    ...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...

16. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...

17. 10 Steps to a Perfect Science Talk

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    ... It makes a great point when I then ask “and who has deposited to the repository?” When most or all of...

18. Thoughts on the Future of Research 2015 Boston Symposium

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    ...Academies define a postdoc as “An individual who has received a doctoral degree (or equivalent) and is...

19. How to Negotiate a Successful Lab Start Up Budget

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    ...means they don’t have the money. If the department has a strict budget, or a lack of resources, then ask...

20. Plasmids 101: E. coli Strains for Protein Expression

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    ... BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression...