We narrowed to 1,022 results for: NTS
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TypeBlog Post...understanding of a GPCR mediated response. Acknowledgements Special thanks to Jeff DiBerto, a graduate...fluorescent protein plasmids Find biosensors for your experiments ...
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An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post...compiles a list of hundreds of different jobs for students to apply to, and after a grueling two months of...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...Like promoter traps, enhancer traps lack enhancer elements and require the trapping of endogenous enhancers...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited...literature, or to decide which line is best for your experiments. You can also use the Cellosaurus STR similarity... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post... over 11,000 conference registrants, including scientists from six continents who dropped by the booth... -
Choosing Your Perfect Empty Backbone
TypeBlog Post.... To help you find the vector that fits your experiments, I've described below some of the most frequently...backbone. Or just ask – leave a message in the comments below and an Addgenie will help you find what ... -
Choosing a Good Mentor for Scientists
TypeBlog Post...scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose... than once to get a full picture and listen for hints at the same concerns from multiple people. Listen... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...jGCaMP7 (keep reading) and will be releasing more variants in the next few months (see “coming soon”). The... can also be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool.... -
Viral Vectors 101: Transductions
TypeBlog Post...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ... -
Summer SciComm: Science Storytelling
TypeBlog Post...communicators also use the hero’s journey, stories from patients or other affected people, man/animal vs nature... the Addgene blog SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...knowledge base to describe aptamers and SELEX experiments. Database, bas006 (2012). PubMed. For a stepwise... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...application is clinical; can we find ways to treat patients? Over a quarter of a million people have some ...activate a set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...for pilot experiments and for interpreting the results of studies. When planning AAV experiments, scientists... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...