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Showing: 821 - 840 of 1015 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase type to use...use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric...

  2. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose... than once to get a full picture and listen for hints at the same concerns from multiple people. Listen...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...

  5. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...jGCaMP7 (keep reading) and will be releasing more variants in the next few months (see “coming soon”). The... can also be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool....

  6. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human...

  8. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated...

  9. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ...

  10. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...communicators also use the hero’s journey, stories from patients or other affected people, man/animal vs nature... the Addgene blog SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...temporal arrangement of chromosomes and how these arrangements may affect nuclear processes. The latest of ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...

  17. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...leading to safer medicine and more successful experiments. It’s something that all scientists should understand...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...feed. Due to increasing prices of methanol and advancements in other biomass production techniques in the...suited to it. Putting K. phaffii to work   The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere...

  19. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...impact on the samples. We therefore devised a few experiments to determine if our tubes were permeable to the... the sample, which may have some effect on the contents of the sample. For example, one study found that...

  20. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...supervisory experience working with undergraduate students? On top of that, perhaps you were involved in ...officers working specifically to assist graduate students in job searching. Start your career planning early...
Showing: 821 - 840 of 1015 results