We narrowed to 838 results for: tat

1. Hot Plasmids - August 2020

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   Blog Post

   ...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

2. Twenty Years of Sharing: Addgene's Viral Vector Service

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   ...to the research community. It does, however, necessitate us first identifying broadly useful vectors, ...

3. How to Be an Excellent Trainee

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   ...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage...

4. Plasmids 101: Shuttle Vectors

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   ...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...

5. CRISPR 101: Any Base Transversion Editors

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   ... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...

6. Twenty Years of Sharing: Expanding Accessibility

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   ...darker blue indicates more views from that country. Stats and figures are helpful, but our favorite measure...

7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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   ... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...

8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ...

9. Viral Vectors 101: Pseudotyping

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   ... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped...

10. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...was certainly an issue worth addressing. Clear expectations have been established for future participants...

11. An Inside Look into Addgene's Dev Team

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    ...provides transparency to everyone involved about the status of each project. This creates accountability on...

12. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...

13. Healthcare Consulting: A Door to the Business of Life Sciences

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    ...pointed feedback or abruptly change needs and expectations. Such situations can get stressful and to help...

14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary...

15. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...intracellular T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic ...

16. Antibodies 101: Beyond Surface Labeling

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    ...cross-linking, killing the cell but preserving the cellular state (think of mummification). Permeabilization, as the...

17. Plasmids 101: Expressing shRNAs for RNA Interference

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    ... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target...

18. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved cameleons."...