We narrowed to 847 results for: tat
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TypeBlog Post...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...
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Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post... on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies to Control Protein Activity... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...scientists' transferrable skills. In describing the limitations of the study, the authors noted that “Many trainees... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...environment Team building events like Escape the Room or Habitat for Humanity take you and your co-workers out of... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments... -
QC Sequencing Technologies at Addgene
TypeBlog Post...time-consuming, depending on the library prep and instrumentation used. Here at Addgene, we sequence every plasmid... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...darker blue indicates more views from that country. Stats and figures are helpful, but our favorite measure... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...to the research community. It does, however, necessitate us first identifying broadly useful vectors, ... -
How to Be an Excellent Trainee
TypeBlog Post...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and... -
Viral Vectors 101: Pseudotyping
TypeBlog Post... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped... -
Hot Plasmids - August 2020
TypeBlog Post...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...was certainly an issue worth addressing. Clear expectations have been established for future participants... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...provides transparency to everyone involved about the status of each project. This creates accountability on... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post... PHP.B and the C57BL/6J version of LY6A, but a mutation in PHP.B’s unique capsid loop disrupted binding... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post... they can understand and are engaged by your presentation, then you’re on the right track. Furthermore...