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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 961 results for: App;

Showing: 421 - 430 of 961 results

  1. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... critical for a number of popular scientific applications, and the quality of a standard curve can make... then generate a trendline for the data. The appropriate trendline varies between assays and may be linear...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95.    Additional... from decreased specificity, especially as it approaches the minimum and maximum thresholds. If the measurements...

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...cells stay in an HDR-promoted cycle longer. This approach may have some negative outcomes on viability if... as 10 hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that...facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is a Cas family...knock your knock-in experiment out of the park. Happy editing!   Resources and References References...

  3. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...down to the exact plasmid you need. The ability to apply a wide range of filters isn’t new to Addgene’s plasmid... it’s also a convenient most requested filter. Applying a second filter, this one from the ‘Requests’ ...consider. If you get this far and realize you need to apply different filters you do not need to start a new...” plasmids returns more than 1300 results, and applying a filter for “CRISPR” plasmids keeps more than...

  4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...activity. In this study scientists examined approximately 1,900 sgRNAs and identified sequence features...substantially identified more hits than the SAM library approach in a comparison study screening for vemurafenib...ORF overexpression library identified several overlapping genes, most genes were unique to the individual...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

  5. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...is a nonsegmented, negative sense RNA strand approximately 19 kb in length. It contains short promoter ...replicated and transcribed in the cell when the appropriate viral proteins are provided in trans (see Other..., there are a few reasons arguing against this approach. First, this would prevent the study of the proteins...activity (4). To ensure that you end up with appropriate plasmid ratios, we suggest: 500 ng NP, 500 ng...

  6. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated knockdown...only infect dividing cells. When choosing the appropriate gene delivery system, the specific target cell...type or tissues; this is useful in gene therapy applications where a distinct subset of the cellular population...free to post them in the comments and we would be happy to answer them! Note: A. Max Juchheim, Marcy Patrick...

  7. Technical Design of a Western Blot

    Type
    Blog Post
    ...it includes validation data relevant to your application — and double-check that lot number while you’...unstained set of standards that covers a size range appropriate for the target(s) and transfer method. Membranes..., make sure to choose a chemilumescence kit appropriately sensitive for the amount of protein you are ...reference table to help you quickly decide what approaches best suit your experimental needs. Table 3: Optimizing...Increased time; increased reagents Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    ... expand, we realized the need to modernize our approach to handle larger volumes of data. In this two-...the need to accelerate science using different approaches. We were also encouraged to see that a community...multiple open-source sequence assembly tools at the appropriate place in our workflow without having to translate...computer ran the process. Another design choice we appreciated about Nextflow was how effortless it was for ...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    .... Transfection of the DNA pooled library and appropriate packaging and envelope plasmids into HEK cells...next step is to make your virus. If the library happens to have a specific protocol for producing virus...protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T cells...don’t hesitate to email us at [email protected]. Happy screening! This blog was written by Addgenie Hannah...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...a danger of getting the disease, even if you do happen to be exposed to asbestos. However, as the U.S....techniques are used as part of a treatment plan, an approach known as multimodal therapy. Sadly, in many cases...the abdominal cavity. This targeted chemotherapy approach has been shown to be successful in killing many...as in the case of Paul Zygielbaum. A different approach to immunotherapy is bevacizumab (Avastin). This...
Showing: 421 - 430 of 961 results