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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,046 results for: ins;

Showing: 421 - 430 of 1046 results

  1. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...single-domain antibodies genetically fused to fluorescent proteins and used to visualize target antigens in living... transgenic mice that express camelid variable domains (aka nanomice), which gives researchers the “best...made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice express...widely adopted. So thank you to the camelids who inspired this versatile family of tools, the applications...

  2. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...and to disrupt endogenous genes at the site of insertion. In both of these cases, transposons combine the...blue) in a plasmid backbone. A separate plasmid contains the transposase gene (red) for expressing the ...sequence is duplicated near the gene of interest insertion site.   The Sleeping Beauty transposon system ...within the genome. The Sleeping Beauty transposase gains its name from the fact that it was recreated from...continues to be altered in order to improve its gene insertion efficiency in primary cells. In 2009, Zsuzsanna...or 5’ end of the gene of interest allowing for insertion in the correct orientation. These plasmids use...

  3. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds LOV2 with high...normally sits on microtubules to attract other proteins involved in their growth and extension. Now, they...domain. Each half was fused with one of the LOVTRAP domains. When both halves of the modified EB1 protein are...inactivated by the separation of its synergistic domains. This paper demonstrated that the migration of ...fluorophore binding aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo...on the lookout for better, brighter fluorescent proteins (FP) with improved tissue penetrance and live ...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...Argonaute proteins are also present in prokaryotes, and some of these proteins protect against invading...reasons why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more! Background...: Argonaute and NgAgo In eukaryotes, Argonaute proteins play an important role in RNA interference, binding...when the protein is being synthesized, so NgAgo remains faithful to its guide DNA and cannot switch guides...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...usage has been limited by technical problems. For instance, it’s much more difficult to introduce a gene ...strategy for transducing primary cells. What if, instead of supplying a DNA construct, they could simply... adapted from D’Astolfo et al. In certain instances, the potential benefits of nonviral transduction...with virus work. Fourth, working directly with proteins removes the limitations of packaging size associated...References Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR...PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional insights." Annu. Rev. Biochem...many molecular biology protocols, including vector-insert joining for recombinant plasmid construction (restriction...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches... can be achieved through a combination of the intrinsic selectivity of the ligase active site and careful...indicated that the optimal buffer for Taq DNA Ligase contains 100 – 200 mM KCl at pH 8.5. This optimization....6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction...

  7. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...accumulate inside the producer cells and purification protocols often describe a process that begins with cell... carried inside the vesicle, which may also contain other cellular molecules such as proteins or nucleic...cells also contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly improve transduction... contexts, such as the delivery of therapeutic proteins to the brain which requires crossing the blood-brain-barrier...

  8. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free extracts Typically...transformation), essentially giving the cell all the instructions it needs to produce that green fluorescent protein...systems contain all the parts needed to produce proteins without the need to handle a living, replicating...simply need to add water to express a variety of proteins. FD-CF reactions are also cost-effective, as each...easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can convert certain...activities fit your lesson plans. If you are an instructor at the college level, perhaps consider using ...

  9. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...but can also be used to brainstorm on data in smaller groups, where for instance the presence of the PI...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new... be written by several authors from different institutes, disciplines, and cultural backgrounds.” This...

  10. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...confirm the presence of an insert in a particular vector by excising the insert from the backbone. To do...use enzymes with restriction sites that flank the insert. You will need to know both the approximate size...vector backbone as well as the predicted size of the insert. You can search NCBI for YGOI to find the particular...right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested with 2 unique enzymes... and 1.2kb (red box), matching the backbone and insert, respectively. The results on the gel correspond...
Showing: 421 - 430 of 1046 results