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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,085 results for: ANT

Showing: 841 - 860 of 1085 results

  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...understanding of the topic will give you some advantage over other candidates. Write about your transferable...research. For example, did you help put together a grant application? Are you familiar with the publishing...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods....

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...γ-retroviruses differ from lentiviruses? There are a few important differences between engineered γ-retroviruses ...names? The nomenclature of the plasmid backbone is meant to be descriptive of the components found within...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...polymerase. E4/E1A activate transcription of other relevant viral genes (including the ones listed previously... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...services for some of the plasmids in our collection. Want to make a vector construct on your own? No problem...

  5. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... a variety of common plasmid features such as antibiotic resistance genes, selectable markers, promoters... files Once you’ve identified a plasmid that you want to learn more about, updates to our sequence analyzer...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...  Multiplexing Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate ...Multiplexing crRNA expression with Cpf1  The key advantage of multiplexing crRNA expression with Cpf1 is ...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...word out about this new resource and providing fantastically useful educational content on viral vectors.... Addgene’s Legal Team acquired permission from relevant plasmid depositors and their institutions to distribute...

  9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins... makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate the activity...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

  12. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research...start using social media to their professional advantage, I’ve selected five resources that have tips for...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...The lab additionally recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

  14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...may seem tedious at first, especially since you’ll want to dig in to the computation, but they will serve...specific goal for each analysis will help you locate relevant information in the future. Approach: Outline...can store any power point, excel, or other files relevant to the project. README: The README.txt document...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...  and that’s just to name a few. But what if you want to study RNA-protein interactions? The characterization...RNAs and proteins with UV light, followed by an antibody pulldown of a protein of interest, much like using...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...

  16. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...riboprobe binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added...stain the cells that contain this mRNA-riboprobe-antibody complex and, as a result, these cells turn blue...Addgene. If you use these published protocols and want to share pictures, feedback etc. please write to...

  18. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...publication.   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans...Dynamics Database (WDDD) The WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

  19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...scientific conference or a karaoke bar), you probably want to sigh. The problem is that many of these studies...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent... directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked (within PAM works, ...

  20. Hot Plasmids - October 2020

    Type
    Blog Post
    ...active in vitro and in human and plant cells. Its small size offers advantages for cellular delivery. Get ...integration and connectivity without the constraints of antibody labeling. This opens the door to in vivo experiments...
Showing: 841 - 860 of 1085 results