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We narrowed to 1,035 results for: CAT

Showing: 841 - 860 of 1035 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...murine members. NRs permit the integration and communication of such signals between central and peripheral...macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa ...

  2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...the most common type of competitive ELISA is a modification of the sandwich ELISA as described here, the...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...

  5. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...accomplishments and demonstrate your science communication skills. Know your audience. Connect your prior...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

  7. Lab Automation at Addgene

    Type
    Blog Post
    ...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...

  8. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...assemblies are possible, the two sets of data together indicate that 35-pieces is a reasonable upper limit for...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...

  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...combined with. Combining a PSAM with the IPD of a cation channel, such as the serotonin 3 receptor, will...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...type are generally broken down into two broad categories, prokaryotic or eukaryotic, based on the functional...termination mechanisms fall under two general categories: rho-dependent and rho-independent. Rho factor...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...particle. Consequently, titers based off of p24 quantification tend to be overestimates since they can include...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...

  13. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...

  14. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...brightness with increasing depth caused by light scattering and absorption.  Finally, the photomultipliers...

  16. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...search to check for expected ORFs, mutations or truncations. A primary advantage of blastx is that you do...other labs speed up their plasmid and cloning verification steps and free up more time for using your plasmids...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and... Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...ribosome may therefore stall at inappropriate locations or fail to make it through the entire transcript...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...
Showing: 841 - 860 of 1035 results