We narrowed to 962 results for: Cre

1. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   ...spinal cord neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that...

2. Bringing Sustainable Practices to the Lab: Recycling

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   ...packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting for 40% of...

3. Build Your CRISPR Vocabulary

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   ...mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM requirements, which...

4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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   ...Figure 1. The RepCap plasmid and its products. Created with BioRender.com   Replication (rep) gene...

5. Antibodies 101: Fc Effector Functions

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   ...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...

6. We're Updating Our Hold for Publication Policy

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   ...plasmids to add the citation and release the hold. Increasing impact Making the plasmids available as soon ...

7. CASTing Off for New Shores in Human Genome Editing

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   ...genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular...

8. Plasmids 101: What is a plasmid?

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   ... foreign DNA into another cell. Minimally, lab-created plasmids have an origin of replication, selection...

9. Antibodies 101: Affinity Tags

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   ...resin. The MBP tag, although large, can actually increase expression and solubility of the tagged protein...

10. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    ...restriction enzymes, but you will also be able to create custom annotations and design additional experiments...

11. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    ... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum...

12. FastCloning: A Newer, Simpler Cloning Technique

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    ... the correct size (vector + insert). You should screen 3-4 colonies to ensure that you find the correct...

13. Management for Scientists: Giving Feedback

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    ...find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ...

14. Advice for Choosing a Research Project

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    ... of combining “new discoveries or technologies increase chances that your project will be fresh as well...

15. The Stingy Scientist: How the Baby Gel Box Was Born

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    ... paying attention to living a “green” life and creating less waste.  Most communities seem to have recycling...

16. Plasmids 101: Antibiotic Resistance Genes

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    ...Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where...

17. Choosing a Good Mentor for Scientists

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    ... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

18. History of CRISPR Cas - A tale of survival and evolution

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    ...genome engineers continue to work diligently and creatively to deliver a highly specific, programmable platform...

19. Viral Vectors 101: Transductions

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    ...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...

20. Antibodies 101: Epitope Tags

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    ...FLAG, but can be more!) to a protein’s termini to increase detection. The tag can also be removed by treating...