We narrowed to 1,028 results for: LEA
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TypeBlog Post... "what is an IgE isotype?" @addgene Ready to learn about antibodies? Our new antibodies animation has...
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Addgene’s First AAV Data Hub Challenge
TypeBlog Post...burning AAV questions answered. Interested? You can learn more here - and remember, submissions are due Nov... -
Help Us Name Blugene’s New Virus Friend
TypeBlog Post...here Additional resources on the Addgene blog Learn more about Blugene's make-over Get a how-to guide... -
CRISPR Guide Update
TypeBlog Post...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog Post...episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology... -
The 12 Days of CRISPR: 2021
TypeBlog Post...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post... nonprofit. Recent remarks and actions by X’s leadership conflict with our values. For this reason, our... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...photos of our pets with Addgene gear, and have unleashed them all over our recently redesigned blog. 🐕... -
CRISPR 101 eBook...en español
TypeBlog Post... Denise Roche, to edit the translation. We are pleased to announce that CRISPR 101: Una herramienta de... -
2021 Deck the Lab Winners!
TypeBlog Post...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though... -
The Twelve Days of CRISPR
TypeBlog Post...to help you with your CRISPR experiments and to learn about new CRISPR tools. Since the CRISPR field keeps... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog Post...difficulties of getting a lab up and running, and learn a little bit about what it was like for Wei Leong... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...create a new fold that is not present in nature (at least that we’ve seen so far!). They started by designing... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...Resources on Addgene.org Check out our Viral Service Learn more about our Viral Production Process Find other... -
Introducing Addgene's Microbiology Pages
TypeBlog Post...information or to start a deposit. If you have a colleague who would benefit from our free distribution services... -
Deck the Lab 2022!
TypeBlog Post...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest! and it's..... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...Channel. Additional resources on the Addgene blog Learn about Addgene's Lab Tips video series Resources... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog Post...management and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing... -
CRISPR Guide
TypeGuide...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating...binding. In this formation, its nuclease domains, RuvC and HNH, cleave opposite strands of the target ...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...Cas9 The two Cas9 nuclease domains, RuvC and HNH, can be altered to reduce cleavage events to a single...contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA. Cas9n ...increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease activity...