We narrowed to 1,016 results for: LEA

...create a new fold that is not present in nature (at least that we’ve seen so far!). They started by designing...

...Resources on Addgene.org Check out our Viral Service Learn more about our Viral Production Process Find other...

...information or to start a deposit. If you have a colleague who would benefit from our free distribution services...

...Channel. Additional resources on the Addgene blog Learn about Addgene's Lab Tips video series Resources...

...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....

...management and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing...

...Your Next Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our...

...for the articles you want to find without even leaving the Addgene blog! Just click on the search bar ...

...Dashboards provide information in a concise and clear way that is accessible by a wide audience. The dashboard...

...reader of science and non-fiction. I just love learning.  This interview was edited for clarity....

...Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the ...

...variety of applications. While editing this post, I learned a lot about how FRET can be used to study biological...

... year we’d like to know what topics you want to learn more about. Our post on “Making Your Own Competent...

...you have information or another resource to add, please tell us about it in the comments. ...

...plasmid cloning Read our Plasmids 101 blog posts Learn about the differences between transformation, transduction...

...links to articles citing this plasmid. Now you can learn how a plasmid has been used by multiple labs and...

... click here to register and join your postdoc colleagues in envisioning the Future of Science. Attend...

... found that our competent cells are good for at least a year when stored at -80oC.   For each new batch...

...'t wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive...

...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating...binding. In this formation, its nuclease domains, RuvC and HNH, cleave opposite strands of the target ...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...Cas9 The two Cas9 nuclease domains, RuvC and HNH, can be altered to reduce cleavage events to a single...contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA. Cas9n ...increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity...