We narrowed to 876 results for: NSI;

1. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   ... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...

2. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   .... They will make mistakes and you will take responsibility (within reason!).  Try to buffer them from ...

3. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...own pros and cons (2). The NgAgo DNA guide is inexpensive and can be 5’-phosphorylated in house using a...

4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   ...think plant biology is massively underfunded considering how important it is. I would really like to reach...

5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   ...multi-spacer CRISPR array. Workflow and technical considerations Making insertions in bacterial genomes with...

6. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...

7. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ...2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list...

8. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   ...fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling...

9. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   ...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page  ...

10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...streptavidin and next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display...

11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

12. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    ...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...

13. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    ...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

14. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    ...unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...

15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ... and model systems. We have used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes...

16. Transferable Skills Guide: Time Management

    Type

    Blog Post

    .... For instance, even if I had planned my day extensively, my lack of desire to go sit in the tissue culture...

17. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    ...different universities are trying to compile more comprehensive demographic information about the postdoc work...

18. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ...cells. For example, KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced...

19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    ...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    ...transformants for appropriately sized inserts. We consider a library worth using if at least 40% of colonies...