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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: PLE;

Showing: 841 - 860 of 1018 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

  3. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...inspires students and teachers to apply engineering principles to biological systems. Today, what started in... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub...

  5. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...change to a slide, think about whether the same principle applies to other slides in your deck.  If someone...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene...double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...several different species are present. One common example is using pre-adsorbed secondary antibodies (which... other antibodies) in experiments that require multiple antibodies that each detect a different antigen...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org...

  11. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... for photoswitchable kinases.  Below are a few examples demonstrated in Zhou et al. 1. Testing kinase ...Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells." Cell ...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

  14. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

  16. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...Overcoming current limitations to permit the use of multiple FRET biosensors simultaneously would further increase...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...academic writing and presentations. But for many people who don’t work in these fields of research, what...

  18. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan...female mouse for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also ...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...
Showing: 841 - 860 of 1018 results