We narrowed to 1,019 results for: REN;
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TypeBlog Post...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...
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Advice for Choosing a Research Project
TypeBlog Post...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know. References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ... -
Antibodies 101: Epitope Tags
TypeBlog Post...Ready to start epitope tagging? References and Resources References Terpe, K., Overview of tag protein... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...studying animals than plants, and biology teachers’ preference for using animal systems to demonstrate biological... a nominal fee for university professors, are currently available through TAIR with a new ABRC database... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...of Medicine Baylor College of Medicine Boston Children's Hospital Brandeis University Broad Institute...Studies The Scripps Research Institute St. Jude Children's Research Hospital Stony Brook University University... -
Plasmids 101: TOPO Cloning
TypeBlog Post...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia... -
Another Pathway into Cells: iTOP
TypeBlog Post...more efficient and feasible for many labs. References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs... -
Hot Plasmids - January 2023
TypeBlog Post...Facility Isotype IgG2a Differences: Distinct variable domains that recognize different epitopes on GFP Monoclonal... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...us at: [email protected] Marco Straccia is currently an Associate Professor at University of Barcelona... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...