We narrowed to 1,045 results for: STI
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TypeBlog Post...zebrafish embryo over an 80 minute time course. By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...
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Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post...of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... strong a signal as with the wild-type AraC, it still reached the level of expression needed for most ...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...have recently received a grant from the National Institutes of Health that will address this problem.The ... represent the official views of the National Institutes of Health. References and Resources References... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...because the secondary antibody is not able to distinguish between the primary antibody and endogenous IgGs...your samples, but know that all is not lost if you still see non-specific staining! Hopefully, these solutions... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post... viral vectors, which are used in the lab, are distinct from viruses — we’ll get to that in a bit! Viral...bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast to ... -
What the HEK?
TypeBlog Post...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells...native channels. No matter what your research question, HEK-293 is known for reproducible results. It... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...world, and the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type of ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...have been able to study the effects of various stimuli on global translation, but a visual technique to... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...stocks and other reagents of interest, the next question to ask is what to do with them. In some cases,... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...methods. Unger plans to use the sensor to answer the question of whether firing of serotonergic neurons (measured... green. A second colonizer, labelled with a constitutively fluorescent reporter can also be used to track... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have..."Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...misinformation about sexual and reproductive health, as the stigma around sexual and reproductive health issues creates...never forgot about all the anxious, whispered questions from friends and classmates back in high school... -
K. phaffii: Rising to the Occasion
TypeBlog Post...either fission or budding, which is the primary distinction between two of the most frequently used yeast...highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because... -
5 Great Apps for Lab Life
TypeBlog Post...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...spectra differ sufficiently for them to be imaged in distinct fluorescent channels on the microscope. With the... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with... -
Advice for Scientists Starting a Lab
TypeBlog Post...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post..., but they do include HEPN nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern... -
Plasmids 101: Inducible Promoters
TypeBlog Post...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...