We narrowed to 937 results for: TIM

...this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged ...

...experiments that require high photostability, such as time-lapse imaging or with high light power detection...

...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...

...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...

...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...

...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...

...genes in particular tissues, but they are costly and time-consuming to generate, especially for large animals...

...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

...contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-...

... maintaining high brightness and fast maturation time (Figure 1). See the paper for more details on small...

...protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to...

...of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...

...expressed in non-dividing cells for long periods of time. This reduces the chance of integration at random...

...before moving onto your experimental samples. With time and practice, you may be able to do your compensation...

...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...

... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts...

...available through Addgene, with more being added all the time. Keep in mind that libraries are only as good as...

...appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental...