We narrowed to 939 results for: TIM

1. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   Blog Post

   ...not be physically present at your poster at all times, so other participants can identify you. You may...

2. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   ...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...

3. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...treat these diseases (drug repositioning). The ultimate goal of this work is to lower the burden of immune...

4. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   ... for viewing how gene expression changes through time or after genetic manipulation. The technique involves...

5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ... interact with the bait at a particular point in time, i.e. when the bait is interacting with a particular...

6. Progress Towards a PAM-Free CRISPR

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   Blog Post

   ...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

7. Hot Plasmids February 2024

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   Blog Post

   ... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

8. Viral Vectors 101: Calcium Sensors

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   ...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...

9. Viral Vectors 101: Virus Safety

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   ...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...

10. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...

11. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...genome of an hiPS cell. While the majority of the time the genome will attempt to repair itself without...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    ...the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet...

13. Plasmids 101: FLEx Vectors

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    Blog Post

    ...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

14. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ...contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-...

15. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts...

16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...

17. Hot Plasmids: Summer 2025

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    Blog Post

    ... maintaining high brightness and fast maturation time (Figure 1). See the paper for more details on small...

18. Degrading DNA with Cascade-Cas3

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    ...of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...

19. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...expressed in non-dividing cells for long periods of time. This reduces the chance of integration at random...

20. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to...