We narrowed to 934 results for: TIM;

1. Hot Plasmids February 2024

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   ... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

2. Viral Vectors 101: Calcium Sensors

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   ...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...

3. Viral Vectors 101: Virus Safety

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   ...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...

4. Progress Towards a PAM-Free CRISPR

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   ...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

5. Antibodies 101: Choosing the Right Isotype

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   ...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...

6. Hot Plasmids and Viral Preps - March 2021

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   ...this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged ...

7. Hot Plasmids and Viral Preps - January 2021

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   ...experiments that require high photostability, such as time-lapse imaging or with high light power detection...

8. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...genes in particular tissues, but they are costly and time-consuming to generate, especially for large animals...

9. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet...

10. Plasmids 101: FLEx Vectors

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    Blog Post

    ...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

11. Plasmids 101: Terminators and PolyA signals

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    ...contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts...

13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...

14. Degrading DNA with Cascade-Cas3

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    ...of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...

15. The AAVantages of AAV in CRISPR Screens

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    ...expressed in non-dividing cells for long periods of time. This reduces the chance of integration at random...

16. Antibodies 101: Flow Compensation

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    ...before moving onto your experimental samples. With time and practice, you may be able to do your compensation...

17. Truncated gRNAs for Regulating Gene Expression

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    ...protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to...

18. Genome-wide Screening Using CRISPR

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    ...available through Addgene, with more being added all the time. Keep in mind that libraries are only as good as...

19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental...

20. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...