We narrowed to 1,082 results for: TOR;
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TypeProtocol... Protocols Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-...enzymes Diagnostic Restriction Digest Protocol Storing and Handling Addgene Plasmids What to do after ...receive your plasmids from Addgene Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization...
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Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...the gene of interest, C-terminal tags, plant terminators and resistance cassettes for selection of transgenic... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post... myself to just simply exist without creating a storm. Given all the options out there, I was certain ... -
Management for Scientists: Seeking Feedback
TypeBlog Post...post, a study coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post... people to connect with you and to share their stories. Thanks to the Benjamin Franklin Effect, most people... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...divided into smaller functional teams. Our Executive Director, Joanne Kamens, responded by starting something... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently have data... -
Science as Art
TypeBlog Post... Geographic and Smithsonian Magazine, where the editor stated “Albert G. Richards, a teacher of dental... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog Post...modified from Ke et al. 2017. 1. Among scientists, historians have the most Twitter users. Biologists rank ... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post... as you will not likely know the exact person monitoring submissions (usually a Human Resources representative... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...is toxic to proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent this ... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...Using CRISPR/Cas to Edit Disease Out of the Genome History of CRISPR Cas - A tale of survival and evolution... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol... mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution ...acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures... room temperature before quantifying and using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits... -
Year of the Camelid: Antibody Style
TypeBlog Post...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol... -
Antibodies 101: Affinity Tags
TypeBlog Post...Ready to start tagging? We are here to help with vectors, protocols, antibodies, and more! Resources ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...nick distance). Both D10A and H840A are potent editors, but their indel profiles vary. In the PAM-out ... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...study how differential access to transcriptional regulatory elements is controlled during development using... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...of leadership. Perhaps one of your advisors or mentors has urged you to work on developing your “leadership... -
Management for Scientists: Giving Feedback
TypeBlog Post...is possible in your eyes so their confidence is restored. A good manager does not hold grudges. Never...