We narrowed to 1,035 results for: URE
-
TypeBlog Post...sharing research materials — we're accelerating the future of science. Want to learn more about our latest...consensus platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095...
-
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post... so be sure to check back often! To find more viral vectors available through Addgene, be sure to check... -
Editor's Choice, September 2016
TypeBlog Post...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights... remember the hazards of that chemical in your obscure growth medium, these apps can help make your lab... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...pooled format at a more-typical scale of tissue culture while still offering the potential for true biological...and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...It could [be used to screen for] morphological features, migration, or survival in different contexts.... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...focus wholly on the science I love. This immersive nature of academia often means that scientists-in-training... video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations... -
Top 10 Open Science Developments of 2013
TypeBlog Post...agencies with over $100 million in annual R&D expenditures to support increased public access to the results... guided by aspirations to promote open science, ensure good stewardship of clinical trial data, serve ... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...based on how each one impacted cell viability (see figure). Most of the mutations were found to be “neutral... gene aberration drivers of pancreatic cancer." Nature communications 7 (2016): 10500. PubMed PMID: 26806015... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...testing multiple gRNAs targeted to the same locus to ensure their effects are specific to that locus. The data...spreadsheet for submitting your gRNA sequences here. Features of the validated gRNA table You can access the... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post... can’t find the molecular cloning guide you were sure was in one of those four white binders. Thirty minutes...) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine. ... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...Plasticity in Mentoring" A recent post on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do ...answers to the other six mentoring questions in future posts. If you have a question about mentoring that... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...targeted with optical stimuli. The restricted light exposure to a specific area in combination with delivery...information from a region. In the example shown in Figure 1, injected retrograde AAVs at one location result... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...complemented each other's strengths: Ginkgo Bioworks’ infrastructure allowed them to synthesize new plasmids quickly...enables science to determine the three dimensional structures of proteins. The RESOLUTE Consortium collection... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...need to be able to replicate in Agrobacterium to ensure that they are maintained during co-cultivation ...plasmid can also be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the ... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...answer to that question. Additionally, a concurrent Nature Biotechnology article describes the development...Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post... a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...265 . https://doi.org/10.1016/j.omtm.2019.01.008 Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics...Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov, K., Van Dort, C.... -
SunTag and Fluorescent Imaging
TypeBlog Post...with the small solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers...the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...Publication policy with two changes that will help us ensure that plasmids don’t get left behind in this status...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and meet... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post... far-eastern islands,” said Tetsushi Sakuma, a lecturer at Hiroshima University who works with Takashi...deposit. Begin yours here. It has been my genuine pleasure to send these small tokens of thanks for the incredible...