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  2. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...with the small solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers...the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ... a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site of...antibiotic-resistance genes conducted in HeLa cells). In their Nature Genetics paper, the lab showed that efficiencies...

  6. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited. Surprisingly...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent ...

  9. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...new. Science involves success and, more often, failure. It’s useful for non-scientists to understand not...science and society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly...

  10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... tool. They can search the database by certain features like serotype, promoter, or tool type and each...use these questions as a guide for designing your future injection experiments. Have your data or lab notebook...

  11. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

  15. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...through biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand...map and cartoon below). Including biotin in your culture and inducing protein expression with IPTG results...

  16. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... been difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE...splice acceptor and donor sites, and introducing premature stop codons.    New from the viral service By...

  17. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...questions help us expand and improve our resources for future users. We’re excited to be distributing new types...fulfilled more than 140 orders for virus! We’ll be sure to keep you updated on our progress and would love...

  19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...interspaced with linkers that can fold into a secondary structure that can bind specific proteins via aptamer domains...using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...
Showing: 841 - 860 of 1019 results