We narrowed to 987 results for: des

1. New and Upcoming Viral Vectors - December 2019

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   Blog Post

   ...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

2. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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   Blog Post

   ...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

3. Plasmids 101: TOPO Cloning

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   Blog Post

   ...needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction...

4. Don’t Wait To Launch Your Health Science Startup

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   Blog Post

   ... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

5. Advice for Moving Into Sales After Your Science Postdoc

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   Blog Post

   ...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...

6. Hot Plasmids: FRET-Based Biosensors

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   Blog Post

   ...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...

7. Mentoring for Scientists: I Have a Mentor, What Now?

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   Blog Post

   ...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

8. Extracurricular Activities for a Strong Science Career Path

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   Blog Post

   ...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

9. What is Polymerase Chain Reaction (PCR)

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   Protocol

   ...Gel Procedure Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...the denaturing. Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set...protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do if my... to work efficiently. Taq Buffer with MgCl 2 : Provides an optimal and stable chemical environment for...

10. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

11. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post

    ...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

12. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post

    ...on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ...

13. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ...bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding...

14. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post

    ...producer cells and purification protocols often describe a process that begins with cell lysis to release...

15. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post

    ...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...

16. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    Blog Post

    ...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...

17. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

    ...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

19. The MTA at Addgene

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    Blog Post

    ...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

20. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post

    ...retroviral CRISPR knockout library using gRNAs designed by the Kosuke Yusa Lab. This retroviral library...