...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction...

... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...

...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...

...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

...oligo overlap cloning, you can design a set of oligos containing your desired restriction sites and add them...that will pay off for years to come. Design Briefly, we will design overlapping oligos that once annealed...procedure will simply differ in terms of primer design). Let's assume that your favorite vector has a ...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...between the EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in...complementary C of the other oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

...gel Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. Basic Molecular...antibody applications. Intro to the Lab Bench Name Description (Link opens in a new window) Link to Video Personal...materials on a balance Basic Molecular Biology Name Description (Link opens in a new window) Link to Video Making...without a commercial kit Plasmid Cloning Name Description (Link opens in a new window) Link to Video Restriction... targeting human and mouse genes. Virus Name Description Link to Video General Transfection Introduce ... your lentivirus preparation Antibodies Name Description Link to Video Transfection Introduce plasmid ...

...on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ...

...bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...

...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

...next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products...

...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...

...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...

...retroviral CRISPR knockout library using gRNAs designed by the Kosuke Yusa Lab. This retroviral library...