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1. Common Injection Routes in Mice

   Type

   Blog Post

   ...of the skin. While this could theoretically be performed anywhere on the body, somewhere large, flat, and...

2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   ... a gene encoding a blue chromoprotein, after transforming them into your cells, you can simply look for...

3. Of Myc and Men

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   Blog Post

   ...Hardly! 9E10’s accessibility and the variety of formats you can find it in likely mean it will remain an...

4. Plasmids 101: Knockout/Knock-In Plasmids

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   ...arms. When we select cells later, we will first perform a positive selection for the antibiotic resistance...

5. Light Sheet Fluorescence Microscopy

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   ...scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged...

6. Plasmids 101: Multicistronic Vectors

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   ...Guide for more molecular biology and cloning information. ...

7. SciComm: Taking Science to Elementary and Middle Schools

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   ...initial guidelines I received from the teachers, I formulated the presentation as follows (which could be adapted...

8. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...this article from The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene...

9. MXS Chaining

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   ...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...number of photons before bleaching. The amount of information one can extract from a biological sample is wholly...

11. Lentiviral Vector Uses and Overview

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    ...titers due to higher plasmid number. For more information on the key differences between the 2nd and 3rd...

12. Cpf1: A New Tool for CRISPR Genome Editing

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    ...overhang 18-23 bases away from the PAM. With this information, they turned to a cell culture system to see ...

13. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...strategy builds on the steps listed above and formalizes your development. You may consider earning a ...

14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...of new technologies may be very effective in transforming conventional research. So, I hope my younger...

15. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...genes in mice. For both genes, they saw indel formation and phenotypic changes as early as 1 week post-injection...

16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...

17. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

19. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

20. Tips for Using FRET in Your Experiments

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    Blog Post

    ...Since then FRET has also been used to measure conformational changes, cleavage activity using FRET-based...