We narrowed to 1,052 results for: sta
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TypeBlog Post...hours of neuronal excitation or inhibition. This sustained activation period is beneficial for use in therapeutic...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...
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SunTag and Fluorescent Imaging
TypeBlog Post...SunTag is expressed at very low levels due to poor stability of the GCN4 scaffold. To increase expression, ...sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4 system does ... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...become a popular model organism because their larval stage lends itself well to studies of neuroscience. The...escape behavior and spontaneous swimming. When startled by acoustic, visual or tactile stimuli, larval... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...the workspace. The color and size of icons are adjustable. New icons can be requested, although there’s... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...Optogenetic Tools Like pDawn Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... order to manage insect pests and to support sustainable agriculture. For this project the expression ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post...open doors to fresh insights and broaden your understanding, ultimately enhancing your project's potential...museums can be excellent methods to detach from the constant stream of thoughts related to lab research and... -
Hot Plasmids - October 2022
TypeBlog Post...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...Find JEDI-2p plasmids here! Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...that I love explaining things to people. When I started my graduate work at Harvard, I recognized that ...are transferable to many different fields. If you start thinking about preparing yourself for a career while... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow... the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described by... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo. ADAs ... -
K. phaffii: Rising to the Occasion
TypeBlog Post...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...well as glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily... -
DAP arrays for multiplex gene editing
TypeBlog Post...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing... writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android This... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding... -
Addgene Depositors Get More Citations
TypeBlog Post... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...