Choosing Your Perfect Empty Backbone
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Blog Post
...antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close...
We narrowed to 1,046 results for: sta
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Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow... the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described by... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...of studying the effects of endogenous looping on distal promoters and enhancers, or have attempted to engineer...these LADL Anchors to genomic targets, such as a distal promoter and an enhancer. What makes LADL unique... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the... -
Addgene Depositors Get More Citations
TypeBlog Post... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...many cases the plasmids themselves are only the starting point for the production of viruses used to deliver...stocks. Our research scientists have provided their standard lentivirus production protocol here and you can... -
DAP arrays for multiplex gene editing
TypeBlog Post...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat... -
K. phaffii: Rising to the Occasion
TypeBlog Post...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...well as glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo. ADAs ... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing... writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android This... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding... -
Components of CRISPR/Cas9
TypeBlog Post...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) ...the different components of CRISPR, It's time to start thinking about how you can used them in your experiments... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ... -
Management for Scientists: Delegating is Key
TypeBlog Post...approachable mistakes will be made that could have been prevented. Make sure everyone understands that no ... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post... and of our data in particular and our deep understanding of Addgene’s customer’s needs. Categories We...engine to be so specialized that it appears to “understand” biology. We hope you will give our new search... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post... are positive for your marker would be a good statistic to compare between samples. In cases where there... immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School... -
Antibodies 101: ChIP
TypeBlog Post...may be thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s ...step can affect the optimization of another. For instance, a sample may need more or less cross-linking ...