We narrowed to 1,055 results for: sta

1. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   ...become a popular model organism because their larval stage lends itself well to studies of neuroscience. The...escape behavior and spontaneous swimming. When startled by acoustic, visual or tactile stimuli, larval...

2. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   Blog Post

   ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...the workspace. The color and size of icons are adjustable. New icons can be requested, although there’s...

3. Hot Plasmids - October 2022

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   ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator...

4. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   ...hours of neuronal excitation or inhibition. This sustained activation period is beneficial for use in therapeutic...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...

5. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   ...are located within their host is often key to understanding their role in both health and disease. To observe...constructs with new acoustic phenotypes. Want to start using acoustic reporters for your research? arg1...

6. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   ...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... order to manage insect pests and to support sustainable agriculture. For this project the expression ...

7. SunTag and Fluorescent Imaging

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   ...SunTag is expressed at very low levels due to poor stability of the GCN4 scaffold. To increase expression, ...sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4 system does ...

8. CASTing Off for New Shores in Human Genome Editing

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   ... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org...

9. Embracing Serendipity: A Crucial Element in the PhD Journey

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   Blog Post

   ...open doors to fresh insights and broaden your understanding, ultimately enhancing your project's potential...museums can be excellent methods to detach from the constant stream of thoughts related to lab research and...

10. Addgene at the ASM Microbe 2016 Conference

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    Blog Post

    ...Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see...

11. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    Blog Post

    ...that I love explaining things to people. When I started my graduate work at Harvard, I recognized that ...are transferable to many different fields. If you start thinking about preparing yourself for a career while...

12. Choosing Your Perfect Empty Backbone

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    Blog Post

    ...antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close...

13. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

    ...about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m...

14. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    ...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow... the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described by...

15. Using Addgene's New Viral Service for Your Research

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    ...repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral...

16. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach...

17. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...of studying the effects of endogenous looping on distal promoters and enhancers, or have attempted to engineer...these LADL Anchors to genomic targets, such as a distal promoter and an enhancer. What makes LADL unique...

18. New and Upcoming Viral Vectors - June 2019

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    ...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...

19. DAP arrays for multiplex gene editing

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    ...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...

20. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post

    ...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo.  ADAs ...