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...that I love explaining things to people. When I started my graduate work at Harvard, I recognized that ...are transferable to many different fields. If you start thinking about preparing yourself for a career while...

...antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close...

...about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m...

...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow... the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described by...

...repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral...

...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach...

...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...well as glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily...

...each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing... writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android  This...

...of studying the effects of endogenous looping on distal promoters and enhancers, or have attempted to engineer...these LADL Anchors to genomic targets, such as a distal promoter and an enhancer. What makes LADL unique...

...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...

...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...

...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo.  ADAs ...

...distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding...

... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

...many cases the plasmids themselves are only the starting point for the production of viruses used to deliver...stocks. Our research scientists have provided their standard lentivirus production protocol here and you can...

...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  ...the different components of CRISPR, It's time to start thinking about how you can used them in your experiments...

...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ...

...approachable mistakes will be made that could have been prevented.  Make sure everyone understands that no ...

...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops, and a bulletin...

...may be thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s ...step can affect the optimization of another. For instance, a sample may need more or less cross-linking ...