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Showing: 841 - 860 of 1044 results

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach...

  7. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...recombinant-deficient bacterial strains such as NEB Stable, Stbl3, JC8111, SURE2, or XL10-Gold will help to...sequences can sometimes be removed to improve vector stability and sequencing results. Next-generation sequencing...

  10. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...well as glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily...

  11. Components of CRISPR/Cas9

    Type
    Blog Post
    ...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  ...the different components of CRISPR, It's time to start thinking about how you can used them in your experiments...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  13. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo.  ADAs ...

  15. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...many cases the plasmids themselves are only the starting point for the production of viruses used to deliver...stocks. Our research scientists have provided their standard lentivirus production protocol here and you can...

  16. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...

  20. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ...
Showing: 841 - 860 of 1044 results