...over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...promising avenues for future engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR...

...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...permeabilize cells rather than to rely on nuclei extraction. They report that this change offers a significant...

...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...

...(get tips for your next science talk). I do illustrations for summarizing mechanisms and applications ...part of Hans’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have...communication with the presenters, and editing of abstracts and speaker biographies.” During the week of ...

...managers are rarely chosen because they have demonstrated skill at managing and this is especially true...getting your PhD or doing a postdoc few science trainees will have opportunities to work on Emotional Intelligence...diversity of responsibilities, which might include training or teaching others, research, and policy development...

...it another way. You’ll know you are on the right track if they start asking you questions. Remember that...out how they got where they are. 8)   Look into training from an organization such as COMPASS. Although...their science matter. If you aren’t ready for a training or one isn’t available yet, order a copy of Nancy...

...Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to turn up the heat when...saving freezer space).  The procedure becomes less attractive when noting that one step involves boiling the... volume.  So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine...

...A scientist-in-training will spend 10 or more years with a small number of formal advisors learning how... will be ones that help them succeed after the training period. While there are many aspects to choosing...choosing a supportive, positive role model. By contrast, as a postdoc, you should choose a field that ...

... sensor AAV GABA is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging...pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between peak fluorescence and resting fluorescence...pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE7c exhibits high contrast between peak fluorescence and resting fluorescence...

...mentors to listen, guide and support us during training and throughout our careers. An easy way to access...every position, we look for candidates that have demonstrated initiative. In fact, hear from Addgene’s former...Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities L’oreal...

...interested in exploring biological dark matter – large tracts of the microbial tree of life that remained unexplored...night was fun but also very tiring. It feels a bit strange to be using a micropipette and gloves at a campsite...rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number...

...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong -...Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical...

...once got a box of 6 perfect, chocolate-covered strawberries for helping with an introduction that helped... have been enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

...information and uses its vast network of articles to track the total number of citations the paper receives...caused by depositing a plasmid with Addgene, in contrast to other reasons for the correlation. However,...Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management. His research...

...; and sequence of biologic product, mode of administration, and dose (Lu et.al., 2020).  Evaluating a ...of therapeutics in silico. These algorithms are trained on vast amounts of data to calculate the relative... immunogenicity is typically performed using traditional 2D assays with immune cell lines or peripheral...

...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...centers provides a potential conflict between traditional IP-based models and the use of taxpayer money...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...

...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...was recognized that an aberrant IgG light chain transcript was expressed by some of the hybridomas and, ...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD...

...fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs can be harnessed ...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...

...than you – meeting scientists at your level of training is always a good idea. Make contact with other... may start in the airport. If you see someone traveling with a poster roll, say hello. Keep your cards...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...Work with a positive, supportive culture, we are attractive to the local employee pool and we get a lot of...inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or a necessary result...