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Showing: 841 - 860 of 1061 results
  1. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...have: working to deadlines, writing up clear and attractive reports, being good at presentations and of course...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...
  2. Hot Plasmids - October 2022

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    Blog Post
    ...over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...promising avenues for future engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR...
  3. Antibodies 101: Affinity Tags

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    Blog Post
    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with... glutathione resin, eliminating the need for an extra clean up step of the tag – a handy feature. If you...fusion proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...
  4. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...(get tips for your next science talk). I do illustrations for summarizing mechanisms and applications ...part of Hans’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have...communication with the presenters, and editing of abstracts and speaker biographies.” During the week of ...
  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post
    ...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...permeabilize cells rather than to rely on nuclei extraction. They report that this change offers a significant...
  6. New CRISPR Web Resources and #12DaysofCRISPR Recap

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    Blog Post
    ...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...
  7. Management for Scientists: What Makes a Good Manager Anyway?

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    Blog Post
    ...managers are rarely chosen because they have demonstrated skill at managing and this is especially true...getting your PhD or doing a postdoc few science trainees will have opportunities to work on Emotional Intelligence...diversity of responsibilities, which might include training or teaching others, research, and policy development...
  8. 10 Ways to Share Your Science!

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    Blog Post
    ...it another way. You’ll know you are on the right track if they start asking you questions. Remember that...out how they got where they are. 8)   Look into training from an organization such as COMPASS. Although...their science matter. If you aren’t ready for a training or one isn’t available yet, order a copy of Nancy...
  9. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post
    ...Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to turn up the heat when...saving freezer space).  The procedure becomes less attractive when noting that one step involves boiling the... volume.  So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine...
  10. Choosing a Good Mentor for Scientists

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    Blog Post
    ...A scientist-in-training will spend 10 or more years with a small number of formal advisors learning how... will be ones that help them succeed after the training period. While there are many aspects to choosing...choosing a supportive, positive role model. By contrast, as a postdoc, you should choose a field that ...
  11. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ... sensor AAV GABA is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging...pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between peak fluorescence and resting fluorescence...pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE7c exhibits high contrast between peak fluorescence and resting fluorescence...
  12. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    Blog Post
    ...mentors to listen, guide and support us during training and throughout our careers. An easy way to access...every position, we look for candidates that have demonstrated initiative. In fact, hear from Addgene’s former...Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities L’oreal...
  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ...; and sequence of biologic product, mode of administration, and dose (Lu et.al., 2020).  Evaluating a ...of therapeutics in silico. These algorithms are trained on vast amounts of data to calculate the relative... immunogenicity is typically performed using traditional 2D assays with immune cell lines or peripheral...
  14. Addgene Depositors Get More Citations

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    Blog Post
    ...information and uses its vast network of articles to track the total number of citations the paper receives...caused by depositing a plasmid with Addgene, in contrast to other reasons for the correlation. However,...Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management. His research...
  15. Celebrate National Book Month with Addgene

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    Blog Post
    ...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong -...Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical...
  16. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...once got a box of 6 perfect, chocolate-covered strawberries for helping with an introduction that helped... have been enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...
  17. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...was recognized that an aberrant IgG light chain transcript was expressed by some of the hybridomas and, ...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD...
  18. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...centers provides a potential conflict between traditional IP-based models and the use of taxpayer money...next few years, with the goal of creating more transparent and reproducible science in the long run (Hohlbein...
Showing: 841 - 860 of 1061 results