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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,085 results for: ANT

Showing: 861 - 880 of 1085 results

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... Zhang’s lab characterized two Cpf1 nucleases, distant cousins of well-known Cas9. Cpf1 cleaves DNA in...2015). PubMed PMID: 26422227.   Potential advantages of Cpf1 Type II CRISPR systems based on Cas9 ...efficiency across CRISPR nucleases and NgAgo is an important next step for the field. Based on what we know...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...with all these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...our CRISPR depositors and requestors. Thank you!  Want to dive deeper into Addgene’s CRISPR data? Read ...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...poster you want to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate...breaths the day-of, you’ll handle them with ease. Anticipate questions: When practicing with your colleagues...

  5. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...researchers were unhappy with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...

  7. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. .... But not all of those 13,000 regions are important for maintaining ESCs. So which of these 13,000 regions...cells compact DNA in a way that brings linearly distant regions close together. gRNA Selection and Filtering...

  8. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9 Type II CRISPR systems based... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... the Addgene repository can save researchers significant time and removes a variable from the lengthy ...from 2016–2023 by type of tool. Equally as important to what is being delivered is where it’s being...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...fluorescent protein control. We also added the toxin-antitoxin stability cassette Axe-Txe so that the plasmid...plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe (Fig...agents to boost expression to appreciable levels. We wanted to create second-generation mARGs with higher expression...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads to everyday...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...learn about the results of studies on reducing antibiotic prescriptions for respiratory tract infections...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    ...biochemical assays followed by in vivo testing. The mantra being “you can’t trust those whacky cell lines”...contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder cancer cells...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing approaches...

  15. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

  16. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...

  18. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

  19. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

  20. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition...
Showing: 861 - 880 of 1085 results