...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...

...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. .... But not all of those 13,000 regions are important for maintaining ESCs. So which of these 13,000 regions...cells compact DNA in a way that brings linearly distant regions close together. gRNA Selection and Filtering...

...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9 Type II CRISPR systems based... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

...continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads to everyday...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...learn about the results of studies on reducing antibiotic prescriptions for respiratory tract infections...

... the Addgene repository can save researchers significant time and removes a variable from the lengthy ...from 2016–2023 by type of tool. Equally as important to what is being delivered is where it’s being...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

...fluorescent protein control. We also added the toxin-antitoxin stability cassette Axe-Txe so that the plasmid...plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe (Fig...agents to boost expression to appreciable levels. We wanted to create second-generation mARGs with higher expression...

...biochemical assays followed by in vivo testing. The mantra being “you can’t trust those whacky cell lines”...contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder cancer cells...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing approaches...

...photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected to a laser...Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become ... The resulting fusion gene is inserted in the antisense orientation with respect to the promoter to prevent...

...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...

...tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition...

...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...her or her family's knowledge. 3. Fossil Rock Anthem One of Tom's earliest and most popular videos. ...

...biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for...for the Addgene blog simply email us. We can’t guarantee that all those who sign up will be able to write...

...References 1. Choi, Vivian W., et al. "Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and...18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great ...

... focusing on safety and the ability to generate mutant microbial strains faster than ever before. 29:54... end : How Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution of new...

...that you can stage in a lab diorama. Or maybe you want to create a stack of wrapped gifts using life science... festivities - however you celebrate! And if you want to see how Addgene celebrates and decorates, follow...