The time and cost required to make a plasmid
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Search for a plasmid in Addgene’s repository
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To Each HIS Own
TypeBlog Post...Much of today's biological research requires a close examination of specific proteins within a system... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...Levine, B. (2010). Methods in Mammalian Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...protocols! We invite feedback from all members of the research community to help us develop these resources further... -
Developing Lab Management Software for Biology
TypeBlog Post...Resources on Addgene.org Find Plasmids for Your Research ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post... viral protocols and videos Find AAVs for your research ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...fluorescence-activated cell sorting (FACS) helps researchers sift through thousands of cells to identify cells... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...reprobe your western blot several times — some researchers report up to ten cycles! Just remember that a... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...alleles, and the growing need for gene editing in research, the old PAM constraints just won’t cut it anymore... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...After all, there’s no sense in spending valuable research dollars on a new secondary antibody if you don... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation with... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...half-lives. Interested in using Split-BioID with your research? Plasmids are available from Addgene! References... -
Lab Automation at Addgene
TypeBlog Post...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...expression in a context-dependent manner. Nucleic Acids Research. 2000. PubMed PMID:10648781. PubMed Central PMCID...