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We narrowed to 1,149 results for: Gene

Showing: 861 - 880 of 1149 results

  1. Summer SciComm: Preprints

    Type
    Blog Post
    ...attendees to read and comment online! Want the general public (say, policymakers or community organizations...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...more openly — which is also our mission here at Addgene! Along with other open-science organizations, we...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

  2. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced in animals...instead of via recombinant DNA, they can’t be genetically tagged and instead are conjugated to reporters...monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody? A RANbody...PubMed PMID: 29440485. Additional Resources on the Addgene Blog Learn more about using the secondary nanobody...Check out this post. Additional Resources on Addgene.org More nanobody expression plasmids can be found...

  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...PMCID: PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...

  5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural affinity and...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

  6. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper published...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...RNA-mediated gene silencing mechanism. It is a useful tool to investigate the roles of specific genes or to ...to knockdown genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian cell...Lentiviral vectors are a preferred method for long-term gene silencing. Once the viral vector is taken up by ...RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods in Molecular Biology, 629 , ...Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral...mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated...

  8. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check out Addgene’s job openings...interviewer,” you can find lists that have lots of general questions about job responsibilities, performance...but then relax.  Additional resources on the Addgene blog: Tips for writing a good cover letter 6 Steps...

  9. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...

  10. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...especially when trying to explore the links between genetics and anatomy, which are not yet fully understood...many different mutants because the Caenorhabditis Genetics Center (CGC) at the University of Minnesota has...Makerspaces like ours can also make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...

  11. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...Overall, spectral flow cytometry has the ability to generate incredible amounts of data from a single experiment...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...

  12. Transferable Skills: Negotiation

    Type
    Blog Post
    ...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  13. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line.     In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot.  The general rules of compensation apply to your experimental...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

  14. Plasmids 101: Protein tags

    Type
    Blog Post
    ... overexpress a recombinant protein? It is not generally in a cell’s best interest to overexpress a protein...for affinity and purification An affinity tag, generally a relatively small sequence of amino acids, is...interest would eventually get ridiculous (you generally don’t want more tag than protein), 2-3 tags is...using plasmids found in this paper (available at Addgene).   Table 2: Protease recognition sites commonly...purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent...

  15. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection... it also has several drawbacks. Direct ELISAs generally have higher levels of background staining. Antigen-containing...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...

  16. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...

  17. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

  18. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ... resources at Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook...sure where to start or what your options are? Addgene's latest animation is here to help! "Antibodies...

  19. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to...and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction...transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...
Showing: 861 - 880 of 1149 results