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We narrowed to 1,106 results for: Ide

Showing: 861 - 880 of 1106 results

  1. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...molecular biology protocols Browse our protocol video collection ...

  2. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally...

  3. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...joined Sanofi/Genzyme nearly three years ago I decided it was time to get in shape. After three kids, ... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link... who is a big superhero fan. This year, I have decided to run as The Green Lantern’s friend, St Walker...

  4. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ... how microbiology research is improving lives worldwide and the presentation made it clear how important...up with former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

  5. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing graduate school, and didn’t ...these days. I do some public speaking and a bit of video work for webinars (get tips for your next science...bring people in.” Are you interested in careers outside academia like science communication? If so, let...

  6. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...HEK293 cells with GeoCas9 or SpCas9 RNP’s and a guide targeting a site with an overlapping PAM. In both...on Your CRISPR Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...

  7. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound. Challenges...experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration and... imaging. Specifically, arg1 expressing E. coli aided in ultrasound imaging of the mouse gastrointestinal...

  8. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...fact, there was so much useful information that I decided to write a few blog posts. This first post will...the box so the pre-paid postage stamp is on the outside of the container, and drop off the box at any location...sustainability leaders. Take initiative to bring ideas to your workplace and you could end up making a ...

  9. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...Publishing Institute (SWPI) is an extremely beneficial guide to becoming a successful writer. When I originally...course The online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy... in Washington D.C. at the ASM headquarters. I decided to go ahead and apply; thinking I probably would...

  10. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...conversations. This creates a level of familiarity and confidentiality to ensure more honest answers. Ask people who...resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this blog post... may hedge on their answers to your questions. Consider reading Toxic Academic Mentors by @drmellivora...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...viruses later on. These built-in safety features provide extra layers of risk management when working with...short-term? Answering these questions will help guide you to the right method for your experiment.  A ...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...of inquiry with which I was not yet familiar. The idea that my piecemeal degree might one day foster a ... several projects nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics... appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable ...

  13. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ....  These days commercially available TOPO kits provide vectors or cloning arms with overhanging 3´ deoxythymidine... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....Molecular Biology Techniques Watch Our Protocol Videos ...

  14. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they...labs looking to improve their AAV yields. Given the wide range of downstream applications scientists rely...resources of the Addgene blog Read our beginner's guide to viral vectors Find all of our viral vector blog...

  15. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...cellular-level events and behavior. It’s a problem that he considers “one of the greatest challenges of neuroscience...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...disease models established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  18. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post
    ...used to infer differentiation trajectories and identify both up- and down-regulated pathways. Individual...unique barcodes for each TF help streamline identification. While the Zhang lab controlled their multiplicity... a model system. TF screens can be designed to identify custom TF programs to acutely initiate disease...

  19. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome...applications when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences...array for multiplex gene editing applications, we provide detailed manual and online design examples of using...

  20. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the mutant allele (7). An independent...CRISPR/Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...
Showing: 861 - 880 of 1106 results