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...plasmid cloning Read our Plasmids 101 blog posts Learn about the differences between transformation, transduction...

...links to articles citing this plasmid. Now you can learn how a plasmid has been used by multiple labs and...

... click here to register and join your postdoc colleagues in envisioning the Future of Science. Attend...

... found that our competent cells are good for at least a year when stored at -80oC.   For each new batch...

...'t wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive...

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...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

... AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here...

... deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the...

....    Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources...

...and easy ways to share your documents with your colleagues. Start small by identifying the protocols, guides...

...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors...

...to observe what’s happening inside cells for at least a couple of decades. Green is the classic color,...

...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity...Learn about different molecular cloning techniques, focusing on seven common cloning methods. Educational...Restriction Enzyme Cloning Restriction enzyme (endonuclease) molecular cloning is the "classic" cloning ...naturally produced by certain bacteria and archaea, cleave double-stranded DNA (dsDNA) at specific sequence...four to 13 base pairs, and produce predictable cleaved ends in the DNA fragments. Given its prevalence...cloning project. Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can...

...recombinant antibodies, so we're partnering with leading neuroscience and antibody experts to expand this...

... an opportunity for the researchers involved to learn techniques from each other, access specialized equipment...

... topic suggestions for our Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick...

...Resources on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR ...