...the next 100 years, many significant discoveries lead to the conclusions that genes encode proteins and...cell division, and each daughter cell receives at least one copy of the plasmid. By the 1970s the combined... tools in the molecular biologist’s toolbox. To learn more about different types of cloning methods check... ( ori ), an antibiotic-resistance gene, and at least one unique restriction enzyme recognition site. ...CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target specific locations...lentiviral particles. Visit our viral service page to learn more. Regardless of type, plasmids are generally...

...'t wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive...

... found that our competent cells are good for at least a year when stored at -80oC.   For each new batch...

...'re accelerating the future of science. Want to learn more about our latest developments? Subscribe to...

... AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here...

...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

... deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the...

....    Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources...

...and easy ways to share your documents with your colleagues. Start small by identifying the protocols, guides...

...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors...

...to observe what’s happening inside cells for at least a couple of decades. Green is the classic color,...

...relevant variants. This list is not exhaustive - please browse our curated list of opsins here to find ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...protein in the light ~400 (on)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible...

... within the past six weeks. Through Addgene, I learned the basics of lab safety, general work skills —...

...CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there are many other Cas proteins as well...

...sequence with one or more enzymes.   Click here to learn how to run an agarose gel     We’ve worked with...

... an opportunity for the researchers involved to learn techniques from each other, access specialized equipment...

... topic suggestions for our Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick...

...Resources on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR ...

...29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase ...