We narrowed to 1,015 results for: LEA
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TypeBlog Post... year we’d like to know what topics you want to learn more about. Our post on “Making Your Own Competent...
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A Scientist's Guide to Ebola
TypeBlog Post...you have information or another resource to add, please tell us about it in the comments. ... -
Optogenetics Guide
TypeGuide...relevant variants. This list is not exhaustive - please browse our curated list of opsins here to find ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...protein in the light ~400 (on)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible... -
Gamma-Retroviral Vector Guide
TypeGuide...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and.... The HEK293T cells will produce the virus and release it into the supernatant. After an initial media...integration sites are found in proto-oncogenes. This can lead to activation of the oncogene and possible development...stimulates the expression of transgenes via increased nuclear export. Psi (Ѱ) in cis RNA packaging signal; recognized...like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material (cDNA... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...plasmid cloning Read our Plasmids 101 blog posts Learn about the differences between transformation, transduction... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post...links to articles citing this plasmid. Now you can learn how a plasmid has been used by multiple labs and... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post... click here to register and join your postdoc colleagues in envisioning the Future of Science. Attend... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog Post...'t wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post... found that our competent cells are good for at least a year when stored at -80oC. For each new batch... -
What's New in CRISPR - December 2019
TypeBlog Post...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post... AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here... -
Reflections and Looking Ahead
TypeBlog Post...'re accelerating the future of science. Want to learn more about our latest developments? Subscribe to... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post... deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the... -
Science as Art
TypeBlog Post.... Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...and easy ways to share your documents with your colleagues. Start small by identifying the protocols, guides... -
Guide to Using Pooled Libraries
TypeGuide...your screen. If you need to amplify the library, please refer to the depositor’s protocol for the best ... library is complete - an incomplete library may lead to false positives or false negatives in later experiments...minimizes the effects of random chance that could lead to false positive or negative results - for a given...sequencing services. For an NGS company near you, please consult these links: NGS Company Resources Science... -
What's New in CRISPR - September 2019
TypeBlog Post...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...to observe what’s happening inside cells for at least a couple of decades. Green is the classic color,... -
Fostering communication in remote collaborations
TypeBlog Post... an opportunity for the researchers involved to learn techniques from each other, access specialized equipment...