We narrowed to 911 results for: Nes

1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   ...the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a...

2. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

3. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   ...science outreach: engaging the public to promote an awareness and understanding of science. Like most science...

4. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   ...you like to bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me...

5. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ...website to verify plasmids or validating your new clones, try these tips to make the most of your time and...

6. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   ... new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction...

7. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   ... of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for each paper...

8. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   ...parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ...Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ...

11. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...place to work, we do SO many fun things!!”  Okay - honestly, maybe not everyone replies with quite so much...

12. How to Be an Excellent Trainee

    Type

    Blog Post

    ...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...

13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    ...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

14. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    ... Common negative controls include knockout cell lines or tissues that do not express the protein. With...

15. Hot Plasmids - August 2020

    Type

    Blog Post

    ...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

16. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    ...pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266...

17. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    ... your gene to be in relation to the rest of the genes? And do you want it to be in the same (forward) ...

18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    ...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...

19. Hot Plasmids - November 2023

    Type

    Blog Post

    ...Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting...

20. Viral Production at Addgene

    Type

    Blog Post

    ... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...