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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: PLE;

Showing: 861 - 880 of 1018 results

  1. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... is reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies...  Multiple Authors...

  2. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...functions in use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries...these categories to see what kinds of CRISPR tools people were depositing and requesting each year (Figure...

  4. Of Myc and Men

    Type
    Blog Post
    ...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    ...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

  8. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  12. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

  13. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

  17. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

  19. New Coomassie Protocol Video

    Type
    Blog Post
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  20. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...
Showing: 861 - 880 of 1018 results