We narrowed to 1,017 results for: PLE;

...functions in use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries...these categories to see what kinds of CRISPR tools people were depositing and requesting each year (Figure...

...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

...transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient...

...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered in his laboratory. Learn...

...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...

...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

... committed to understanding some of the basic principles of life and health and works to advance foundational...enhancer sequence, then interact with mediator complexes and the RNA polymerase at the promoter region ...

...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

...modifications. CRISPR base editing persists through multiple cell divisions, indicating that this method produces... Multiple Authors...

...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...