We narrowed to 1,014 results for: REN;
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TypeBlog Post...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse. References 1. Tervo, D. Gowanlock R., et al. "A designer...
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Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ... -
Hot Plasmids - May 2022
TypeBlog Post...peptide. By combining three different localization peptides with four different fluorescent proteins, and... -
Antibodies 101: ChIP
TypeBlog Post...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey! References and Resources More resources on Addgene’s blog... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania... -
Visualizing Protein Turnover In Situ
TypeBlog Post...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... -
Addgene: The First Twenty Years
TypeBlog Post...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...transfer, each GOI is usually expressed from a different plasmid, which may lead to unequal gene dosage...consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all possible combinations... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant... -
Developing Lab Management Software for Biology
TypeBlog Post...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research! References 1.Tang, Jonathan C.Y., et al. “A nanobody-based... -
Plasmids 101: Cre-lox
TypeBlog Post...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques? Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang...dCas9-CBP and the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang... -
Viral Vectors 101: Virus Safety
TypeBlog Post...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance...