We narrowed to 1,019 results for: REN;
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TypeBlog Post...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...
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How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...the interview process was several short talks by current faculty members and it just blew me away…. I have...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to... -
Finding and Joining Your Dream Lab
TypeBlog Post...interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse. References 1. Tervo, D. Gowanlock R., et al. "A designer... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of... -
Hot Plasmids - May 2022
TypeBlog Post...peptide. By combining three different localization peptides with four different fluorescent proteins, and... -
Antibodies 101: ChIP
TypeBlog Post...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey! References and Resources More resources on Addgene’s blog... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania... -
Addgene: The First Twenty Years
TypeBlog Post...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the... -
Visualizing Protein Turnover In Situ
TypeBlog Post...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...transfer, each GOI is usually expressed from a different plasmid, which may lead to unequal gene dosage...consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all possible combinations... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant... -
Developing Lab Management Software for Biology
TypeBlog Post...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research! References 1.Tang, Jonathan C.Y., et al. “A nanobody-based...