We narrowed to 1,056 results for: STI

...have been able to study the effects of various stimuli on global translation, but a visual technique to... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing...

...stocks and other reagents of interest, the next question to ask is what to do with them. In some cases,... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ...

...methods. Unger plans to use the sensor to answer the question of whether firing of serotonergic neurons (measured... green. A second colonizer, labelled with a constitutively fluorescent reporter can also be used to track...

...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have..."Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors...

...misinformation about sexual and reproductive health, as the stigma around sexual and reproductive health issues creates...never forgot about all the anxious, whispered questions from friends and classmates back in high school...

...either fission or budding, which is the primary distinction between two of the most frequently used yeast...highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because...

...spectra differ sufficiently for them to be imaged in distinct fluorescent channels on the microscope. With the... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with...

...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers...

...advice for using Twitter, this post provides suggestions for using LinkedIn and Facebook. For each of ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...

..., but they do include HEPN nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

... development, organs of interest, such as the intestines, can be dissected at various times throughout... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. J. ...

... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...

... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...

...fluorescent to the surface of the bead, suggesting that OptoNBs still bound their target protein even when...

...gold standard in gene expression analysis to investigate the effect of regulatory elements — such as promoters...interaction occurs, the luciferase emits its characteristic light. However, if a protein interaction brings...

..., B-B’, and C-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

...making virus’ or what gag and pol stand for, we demystify all components of viral vectors here! Need Virus...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

...producing the proteins – are not, and many of the existing GCE tools are not very robust. When using GCE,...work. Plus, keep an eye on our blog – we’ll be posting more posts on the various protocols and helping...

...each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction...