We narrowed to 931 results for: TIM

... that a construct is functional before investing time and resources into making a stable cell line. When...

...that will work well with a given set of resources, time, and experimental needs. Since its invention in ...

...experiments. They’ve also helped researchers design and optimize cloning methodologies using counterselection. ...

...libraries Overview of Library To better define the optimal conditions for CRISPR knockout screens, the Bassik...

...Gail Mandel and her team have shown for the first time that programmable RNA editing can repair a gene ...

...by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors...

...peripheral nervous system (PNS) targeting AAV  Get 1st time AAV user tips from an AAV rookie  Learn about some...

...and Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an...

...annotated like so: CD14high or CD14low.  Don’t be intimated if you’re struggling to understand how the researchers...

...AIBS). Scientists at AIBS have allocated tremendous time and resources to generate highly specific tools ...

...vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...

...lentiviral minus strand in the 3’ LTR. These optimizations produced 90% accurate integration of multiplexed...

...episomal vector system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding...

...appropriate isoform(s) of a given gene, as, many times, the isoform(s) common to a particular cell type...

...in vivo applications.  Third, even in the most optimized tissue-culture based system, the efficiency of...

...dedicated field of study for improving these tools. Next time you need to knock down a gene, maybe you’ll consider...

...using biorender.com.   Intracellular labeling Oftentimes, a marker of interest is not located on the cell...

...(McInnes et al., 1990). Photobiotinylation is sometimes easier and less expensive than other enzymatic...

...genomes will need to be sequenced to determine the optimal gRNAs for either “shock and kill” or viral excision...

...designing your plasmid – you carefully selected the optimal promoter for your gene of interest, painstakingly...