We narrowed to 939 results for: TIM

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts...

2. Genome-wide Screening Using CRISPR

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   ...available through Addgene, with more being added all the time. Keep in mind that libraries are only as good as...

3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental...

4. An Introduction to Adenovirus

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   ...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

5. Plasmids 101: Secondary Nanobody Toolbox

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   ...antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit...

6. CRISPR 101: RNA Editing with Cas13

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   ...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

7. Uncovering Ancestral Bias in CRISPR Research

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   ...ancestry group. With the existing methodology, they estimate that the African ancestry-associated cancer dependencies...

8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...titration experiments might be helpful to achieve optimal results. It is important to note that this system...

9. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ... that a construct is functional before investing time and resources into making a stable cell line. When...

10. Plasmids 101: Gateway Cloning

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    ...that will work well with a given set of resources, time, and experimental needs. Since its invention in ...

11. Hot Plasmids Spring 2024

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    ...lentiviral minus strand in the 3’ LTR. These optimizations produced 90% accurate integration of multiplexed...

12. Viral Vectors 101: Pseudotyping

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    ...by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors...

13. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...episomal vector system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding...

14. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...and Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an...

15. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...experiments. They’ve also helped researchers design and optimize cloning methodologies using counterselection. ...

16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...libraries Overview of Library To better define the optimal conditions for CRISPR knockout screens, the Bassik...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...peripheral nervous system (PNS) targeting AAV  Get 1st time AAV user tips from an AAV rookie  Learn about some...

18. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...AIBS). Scientists at AIBS have allocated tremendous time and resources to generate highly specific tools ...

19. Plasmids 101: Shuttle Vectors

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    ...vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...

20. Antibodies 101: Reading a Flow Plot

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    ...annotated like so: CD14high or CD14low.  Don’t be intimated if you’re struggling to understand how the researchers...