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  2. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... Julie from Novartis who was working with cell culture and who was extremely helpful since she was familiar...useful pieces of information from the workshop. Future workshops for Addgene Addgene strives to be a great...

  3. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...? Check Out Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...with each other. Below we will outline the common features as well as the differences within their viral ...Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and Function. Viruses. 2021 13(3):388. DOI:10.3390...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal vector ...low immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information in ...resources from the Addgene blog Learn about other features on Addgene's website Find a step-by-step guide...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...lentiCas9-blast to generate Cas9-expressing cells. To ensure that this growth rate effect doesn’t confound your... that express cre recombinase. Typically, this feature is used to attain cell-specific expression in transgenic...

  11. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express your...immediate and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...

  16. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

  18. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

  20. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...
Showing: 861 - 880 of 1020 results