...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to...

...of R bodies R bodies have been studied for many decades, and they were known to be resistant to detergents...

...Define the scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab ...

...University of British Columbia, Student Services - includes videos on general resume writing, as well as additional...

...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate to ...

...different phenotypes. For example, this plasmid encodes a green fluorescent protein gene which makes the...

... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

...even if science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often...

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh...

...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

...There are four biosafety levels. This protocol provides information for both biosafety level 1 (BSL-1)...level has different safety requirements. BSL-1 is designated for those working with microbes that don’t cause...Staphylococcus aureus or Vibrio cholerae . BSL-2 includes all of the precautions needed in BSL-1, along ...before and after working in the lab. Ensure that a designated chemical waste accumulation site is present, ...(MSDS). While You Work Dispose of sharps in a designated sharps container. Sharps include anything that... drain. Solid BSL-2 waste can be collected in designated biohazardous waste containers that can be autoclaved...important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and...

... Procedure Design your plasmid and order primers (see figure to the right). When designing your plasmid...and enrich for correctly assembled plasmids by designing primers to split an antibiotic resistance gene...and yield. If there are significant amounts of undesired product, gel-purify DNA segments. Otherwise, PCR... High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed DNA fragments... (<150 bp). Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers...window) . NEB has other resources, such as a primer design tool (Link opens in a new window) . Gibson Assembly...

...created. Additional design rules can be found at the Broad Institute's TRC shRNA Design Process . To minimize...Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal...sites in place of the stuffer. The AgeI site is destroyed in most cases (depending on the target sequence....9kb stuffer, visit www.addgene.org/10878/ . Description Vector Element U6 Human U6 promoter drives RNA...the pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector ...website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos for pLKO.1 B.1 Determining the Optimal...accessed after a free registration. Guidelines for designing siRNAs with effective gene silencing: Starting...