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TypeBlog Post...genes in mice. For both genes, they saw indel formation and phenotypic changes as early as 1 week post-injection...
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"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...Genetic Tools Atlas (GTA). The GTA has a wealth of information and includes annotated images of the brain sections... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology... -
Tips for Using FRET in Your Experiments
TypeBlog Post...Since then FRET has also been used to measure conformational changes, cleavage activity using FRET-based... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...proteins from a complex of interest. For more information, read the Split-BioID blog post. Find plasmids... -
Five Popular Model Organisms
TypeBlog Post...extrachromosomal maintenance and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post... very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use the system to ... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...Iwakawa, H., & Tomari, Y. (2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...find personnel salary ranges, go to online job information sites (like, indeed.com, or salary.com). Search... -
Plasmids 101: Repressible Promoters
TypeBlog Post...recent variants of the Tet-Off system. For more information on Addgene Tet plasmids, see our Tetracycline... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...technology. Read the genOway press release for more information. References Abdallah, Khaled, et al. "Adeno-associated... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting in processing... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...nodes. These methods gave researchers much more information regarding immune cell mobility than the static... -
Typing CRISPR Systems
TypeBlog Post...Class 2 CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate and redifferentiate... -
Immunology Research Plasmids and Resources
TypeCollection...subunit B, beta isoform PPP3RL PRF1 perforin 1 (pore forming protein) FLH2, HPLH2, MGC65093, P1, PFN1,...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform CALNA3 PPP3R1 protein phosphatase 3 (formerly 2B),...PPP3CA protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, ...PPP3CB protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform CALNA2, CALNB PPP3CC protein... -
Viral Production
TypeCollection...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control.... To learn which specific QC experiments were performed on your lot, please contact us . Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection... the following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus... CA2 and Fasciola cinerea neurons Hippocampal formation 214506 pAAV-AiE2333m-minBG-SYFP2-WPRE3-BGHpA AiP1653...AiE2333m SYFP2 CA3 pyramidal neurons Hippocampal formation 220731 pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA AiP2141...AiE2333m Cre CA3 pyramidal neurons Hippocampal formation 214552 pAAV-AiE2435m-minBG-SYFP2-WPRE3-BGHpA AiP1848...AiE2435m SYFP2 Cajal-Retzius neurons Hippocampal formation 214597 pAAV-AiE2563m-minBG-SYFP2-WPRE3-BGHpA AiP1969...Entorhinal area - L2 pyramidal neurons Hippocampal formation 214539 pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA AiP1772...Entorhinal area - L2 pyramidal neurons Hippocampal formation 230553 pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA AiP2107... -
AAV Packaged on Request
TypeCollection...of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI...AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more information before choosing a serotype? Learn more about ... can do this whenever you are ready. Payment information must be added before we can begin processing ...this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes to make...Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping time will...whether the item can be packaged. Addgene does not perform functional or in vivo testing. We recommend that...Addgene’s Help Center, a searchable database of information to help you every step of the way, including ...