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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 861 - 880 of 960 results

  2. Five Popular Model Organisms

    Type
    Blog Post
    ...extrachromosomal maintenance and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959...

  10. Typing CRISPR Systems

    Type
    Blog Post
    ...Class 2 CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...subunit B, beta isoform PPP3RL PRF1 perforin 1 (pore forming protein) FLH2, HPLH2, MGC65093, P1, PFN1,...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform CALNA3 PPP3R1 protein phosphatase 3 (formerly 2B),...PPP3CA protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, ...PPP3CB protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform CALNA2, CALNB PPP3CC protein...

  13. Viral Production

    Type
    Collection
    ...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...Link opens in a new window) . Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control.... To learn which specific QC experiments were performed on your lot, please contact us . Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation...

  14. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ... the following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus... CA2 and Fasciola cinerea neurons Hippocampal formation 214506 pAAV-AiE2333m-minBG-SYFP2-WPRE3-BGHpA AiP1653...AiE2333m SYFP2 CA3 pyramidal neurons Hippocampal formation 220731 pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA AiP2141...AiE2333m Cre CA3 pyramidal neurons Hippocampal formation 214552 pAAV-AiE2435m-minBG-SYFP2-WPRE3-BGHpA AiP1848...AiE2435m SYFP2 Cajal-Retzius neurons Hippocampal formation 214597 pAAV-AiE2563m-minBG-SYFP2-WPRE3-BGHpA AiP1969...Entorhinal area - L2 pyramidal neurons Hippocampal formation 214539 pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA AiP1772...Entorhinal area - L2 pyramidal neurons Hippocampal formation 230553 pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA AiP2107...

  15. Antibody Production

    Type
    Collection
    ...methods, and quality control are described below. Information for a specific antibody can be found on the antibody's...reviewing the specific antibody webpage for further information. Production For recombinant antibodies (rAb) ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the.... To learn which specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry...

  16. AAV Packaged on Request

    Type
    Collection
    ...of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI... can do this whenever you are ready. Payment information must be added before we can begin processing ...this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes to make...Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping time will...whether the item can be packaged. Addgene does not perform functional or in vivo testing. We recommend that...Addgene’s Help Center, a searchable database of information to help you every step of the way, including ...

  17. Rett Syndrome

    Type
    Collection
    ...literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information (Link...which they were created. Find their contact information by following the link to their laboratory website...Link opens in a new window) Wendy Gold For more information on generating iPSCs using plasmids, please see...MECP2 - MECP2 curated protein and functional information Mouse and Cell Line Repositories (Link opens ...Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH image data...

  18. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available to download...window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to assay...techniques. Using a single protein with many available isoforms and mutants provides a shared experience for the...mentor, and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student...

  19. Open Enzyme Collection

    Type
    Collection
    ...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...genetic engineering and directed evolution. More information about the collection can be obtained at the Open...an expression vector of their choice and then transform into E. coli for expression and purification (... in this collection is ampicillin. Find more information on the enzymes and protocols for expression in...Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 PGD T4 PGD...

  20. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Packaged on Request Viral Vector Packaging Service Information Production Our production process and quality...viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources ...from the research community demonstrating AAV performance under various experimental conditions. Protocols...institutional biosafety office and your lab, information on the biosafety of viral vectors, and general...
Showing: 861 - 880 of 960 results