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Showing: 861 - 880 of 996 results
  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...
  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot...-term protein expression observed using transient transfection approaches, generating cell lines using...experiments in a stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it... the variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...
  3. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...
Showing: 861 - 880 of 996 results