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We narrowed to 1,046 results for: sta

Showing: 861 - 880 of 1046 results

  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops, and a bulletin...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...only 44% and 66% indel frequency with the use of standard single gRNA constructs. When PTG constructs with...<200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...intervening DNA (“pop-out” deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to...References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence of a Chromosome...

  5. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...several considerations. First, it is important to establish the type of audience you want to reach with your...and help you correct any language- and spelling mistakes. A manuscript that is well written looks more ...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect ...interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction detection ...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...resulting in GFP signal. With the addition of standards, the assay is semi-quantitative and can approximate...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    ...However, transcription of thousands of genes is constantly occurring throughout a single cell, so these ... these structures can in turn lead to genome instability. Thus, it’s important to strike a balance between...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    ...possible antibody conjugates (especially if you start to consider clinical applications). But there are...make the process quick and easy. Before getting started, be sure to check that your antibody’s buffer is...

  11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...that treat disease at the molecular level. For instance, if a disease is caused by a mutation in a single...developing AAV-based therapies. Furthermore, deeper understanding of disease pathogenesis will enable robust therapeutic...

  12. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...use electroporation, a technique that briefly destabilizes the cell’s outer membrane and allows the components...structures and processes and can lead to the better understanding of cell biology. We hope that these high-quality...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position and is ...employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used to flip...

  17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...light up endogenous genes of interest to better understand normal protein function. However, CRISPR protein...introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and...

  19. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...number of neurons overall (GFP+ cells that also stained for a neuronal marker). Check out tables 1 and ...

  20. MXS Chaining

    Type
    Blog Post
    ... (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed by ligation...Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological part assembly. Journal of biological...
Showing: 861 - 880 of 1046 results