...the embryos underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma...maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

...Melina Fan, a cell biology graduate student, was frustrated by the difficulty of accessing published plasmids...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...

...make your own antibody fragments as needed.  In contrast, anti-IgG antibodies, commonly used as secondary...’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’ can act as a convenient... expression and will have the highest tissue penetration. However, they tend to be less stable and have...

...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...Work with a positive, supportive culture, we are attractive to the local employee pool and we get a lot of...inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or a necessary result...

...expressed in transfected eukaryotic cells, allowing for them to be used as intracellular inhibitors.  ...Smaller, slimmer affinity reagents can have deeper penetration into a tissue, useful for applications like IHC...

...institutional subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues... the action. If the paper is well-written, the abstract should give you a sense of the importance of the... the dark ages with respect to my organization strategy. There are a number of software options for helping...

...myasthenic syndrome (LEMS) because, at high concentrations (25 mM), Gdm+ binds to and inhibits a potassium...living cells. The McMurray lab found that low concentrations (≤3 mM) of GdnHCl can functionally rescue multiple...available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl chemical rescue of a variety of disease-linked...

...other words, newborn poop) visible through their translucent bodies. This is the ideal stage to select for...recombination can potentially cause you to lose track of your mutation. To maintain stable lines of flies... transgenic flies...

...schedule. If there is a list of poster titles or abstracts available before the conference, comb through ...notes or questions from your poster viewers and trade contact information if your conversation is particularly...overwhelming when you are presented with a sea of strangers. Try things like sitting at a table for lunch ...

...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...differentiation. Using a cell line model, the Xu Lab tracked enhancer-promoter interactions during erythroid...

...fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an intercalating dye, with... the readout gives an absolute value of DNA concentration. By comparing the number of positive droplets...gene, copy number can be determined through concentration values, rather than by comparison to a standard...

...of current new and exciting literature. As I transitioned out of academia and away from the bench I certainly... decide on the topic, there are a number of administrative and practical decisions to make. How often ...presenter, figure by figure) if the primary purpose is training graduate students in critical reading. If the ...

...has grown, it has taken effort to continue the tradition and plan enjoyable events for employees. We now..., evite, posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of...people excited!    Laura Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition...

...usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon...peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...

...need to care for an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper...have time to read a story to your baby or run that extra two miles which is way more fun. And despite those...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...professor or hiring manager. Otherwise, use a gender-neutral greeting like “Dear Hiring Manager.” Highlight ...

...When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic gRNA) are derived from...formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation...Christie KA, Whittaker MN, Kleinstiver BP (2020) Unconstrained genome targeting with near-PAMless engineered...

...focus on you and your ideas. Slides will be     distracting. What is the big question? This should be...interview, your previous success will not necessarily translate into getting a job. Your papers and pedigree get...full eBook (available here for free!) contains strategies, hints, anecdotes, and insights. After I wrote...

...were even able to have many of our remote workers travel to Addgene's headquarters in Watertown, Massachusetts...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...

...One of our favorite holiday traditions is our annual Twelve Days of CRISPR campaign! But if you missed...eighth day of CRISPR, Addgene gave to me: the PEDAR strategy to delete and replace long genomic sequences. ...